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Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission

Mitochondria exist in an equilibrium between fragmented and fused states that shifts heavily toward fission in response to cellular damage. Nuclear-to-cytoplasmic cyclin C relocalization is essential for dynamin-related protein 1 (Drp1)–dependent mitochondrial fission in response to oxidative stress...

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Autores principales: Ganesan, Vidyaramanan, Willis, Stephen D., Chang, Kai-Ti, Beluch, Samuel, Cooper, Katrina F., Strich, Randy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589575/
https://www.ncbi.nlm.nih.gov/pubmed/30516433
http://dx.doi.org/10.1091/mbc.E18-07-0463
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author Ganesan, Vidyaramanan
Willis, Stephen D.
Chang, Kai-Ti
Beluch, Samuel
Cooper, Katrina F.
Strich, Randy
author_facet Ganesan, Vidyaramanan
Willis, Stephen D.
Chang, Kai-Ti
Beluch, Samuel
Cooper, Katrina F.
Strich, Randy
author_sort Ganesan, Vidyaramanan
collection PubMed
description Mitochondria exist in an equilibrium between fragmented and fused states that shifts heavily toward fission in response to cellular damage. Nuclear-to-cytoplasmic cyclin C relocalization is essential for dynamin-related protein 1 (Drp1)–dependent mitochondrial fission in response to oxidative stress. This study finds that cyclin C directly interacts with the Drp1 GTPase domain, increases its affinity to GTP, and stimulates GTPase activity in vitro. In addition, the cyclin C domain that binds Drp1 is contained within the non–Cdk binding second cyclin box domain common to all cyclin family members. This interaction is important, as this domain is sufficient to induce mitochondrial fission when expressed in mouse embryonic fibroblasts in the absence of additional stress signals. Using gel filtration chromatography and negative stain electron microscopy, we found that cyclin C interaction changes the geometry of Drp1 oligomers in vitro. High–molecular weight low–GTPase activity oligomers in the form of short filaments and rings were diminished, while dimers and elongated filaments were observed. Our results support a model in which cyclin C binding stimulates the reduction of low–GTPase activity Drp1 oligomers into dimers capable of producing high–GTPase activity filaments.
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spelling pubmed-65895752019-06-28 Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission Ganesan, Vidyaramanan Willis, Stephen D. Chang, Kai-Ti Beluch, Samuel Cooper, Katrina F. Strich, Randy Mol Biol Cell Brief Reports Mitochondria exist in an equilibrium between fragmented and fused states that shifts heavily toward fission in response to cellular damage. Nuclear-to-cytoplasmic cyclin C relocalization is essential for dynamin-related protein 1 (Drp1)–dependent mitochondrial fission in response to oxidative stress. This study finds that cyclin C directly interacts with the Drp1 GTPase domain, increases its affinity to GTP, and stimulates GTPase activity in vitro. In addition, the cyclin C domain that binds Drp1 is contained within the non–Cdk binding second cyclin box domain common to all cyclin family members. This interaction is important, as this domain is sufficient to induce mitochondrial fission when expressed in mouse embryonic fibroblasts in the absence of additional stress signals. Using gel filtration chromatography and negative stain electron microscopy, we found that cyclin C interaction changes the geometry of Drp1 oligomers in vitro. High–molecular weight low–GTPase activity oligomers in the form of short filaments and rings were diminished, while dimers and elongated filaments were observed. Our results support a model in which cyclin C binding stimulates the reduction of low–GTPase activity Drp1 oligomers into dimers capable of producing high–GTPase activity filaments. The American Society for Cell Biology 2019-02-01 /pmc/articles/PMC6589575/ /pubmed/30516433 http://dx.doi.org/10.1091/mbc.E18-07-0463 Text en © 2019 Ganesan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Brief Reports
Ganesan, Vidyaramanan
Willis, Stephen D.
Chang, Kai-Ti
Beluch, Samuel
Cooper, Katrina F.
Strich, Randy
Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title_full Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title_fullStr Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title_full_unstemmed Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title_short Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission
title_sort cyclin c directly stimulates drp1 gtp affinity to mediate stress-induced mitochondrial hyperfission
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589575/
https://www.ncbi.nlm.nih.gov/pubmed/30516433
http://dx.doi.org/10.1091/mbc.E18-07-0463
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