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Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish

The precise spatial and temporal expression of genes is essential for proper organismal development. Despite their importance, however, many developmental genes have yet to be identified. We have determined that Fer1l6, a member of the ferlin family of genes, is a novel factor in zebrafish developme...

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Autores principales: Bonventre, Josephine A., Holman, Chelsea, Manchanda, Aayushi, Codding, Sara J., Chau, Trisha, Huegel, Jacob, Barton, Carrie, Tanguay, Robert, Johnson, Colin P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589578/
https://www.ncbi.nlm.nih.gov/pubmed/30516436
http://dx.doi.org/10.1091/mbc.E18-06-0401
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author Bonventre, Josephine A.
Holman, Chelsea
Manchanda, Aayushi
Codding, Sara J.
Chau, Trisha
Huegel, Jacob
Barton, Carrie
Tanguay, Robert
Johnson, Colin P.
author_facet Bonventre, Josephine A.
Holman, Chelsea
Manchanda, Aayushi
Codding, Sara J.
Chau, Trisha
Huegel, Jacob
Barton, Carrie
Tanguay, Robert
Johnson, Colin P.
author_sort Bonventre, Josephine A.
collection PubMed
description The precise spatial and temporal expression of genes is essential for proper organismal development. Despite their importance, however, many developmental genes have yet to be identified. We have determined that Fer1l6, a member of the ferlin family of genes, is a novel factor in zebrafish development. We find that Fer1l6 is expressed broadly in the trunk and head of zebrafish larvae and is more restricted to gills and female gonads in adult zebrafish. Using both genetic mutant and morpholino knockdown models, we found that loss of Fer1l6 led to deformation of striated muscle tissues, delayed development of the heart, and high morbidity. Further, expression of genes associated with muscle cell proliferation and differentiation were affected. Fer1l6 was also detected in the C2C12 cell line, and unlike other ferlin homologues, we found Fer1l6 expression was independent of the myoblast-to-myotube transition. Finally, analysis of cell and recombinant protein–based assays indicate that Fer1l6 colocalizes with syntaxin 4 and vinculin, and that the putative C2 domains interact with lipid membranes. We conclude that Fer1l6 has diverged from other vertebrate ferlins to play an essential role in zebrafish skeletal and cardiac muscle development.
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spelling pubmed-65895782019-06-28 Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish Bonventre, Josephine A. Holman, Chelsea Manchanda, Aayushi Codding, Sara J. Chau, Trisha Huegel, Jacob Barton, Carrie Tanguay, Robert Johnson, Colin P. Mol Biol Cell Brief Reports The precise spatial and temporal expression of genes is essential for proper organismal development. Despite their importance, however, many developmental genes have yet to be identified. We have determined that Fer1l6, a member of the ferlin family of genes, is a novel factor in zebrafish development. We find that Fer1l6 is expressed broadly in the trunk and head of zebrafish larvae and is more restricted to gills and female gonads in adult zebrafish. Using both genetic mutant and morpholino knockdown models, we found that loss of Fer1l6 led to deformation of striated muscle tissues, delayed development of the heart, and high morbidity. Further, expression of genes associated with muscle cell proliferation and differentiation were affected. Fer1l6 was also detected in the C2C12 cell line, and unlike other ferlin homologues, we found Fer1l6 expression was independent of the myoblast-to-myotube transition. Finally, analysis of cell and recombinant protein–based assays indicate that Fer1l6 colocalizes with syntaxin 4 and vinculin, and that the putative C2 domains interact with lipid membranes. We conclude that Fer1l6 has diverged from other vertebrate ferlins to play an essential role in zebrafish skeletal and cardiac muscle development. The American Society for Cell Biology 2019-02-01 /pmc/articles/PMC6589578/ /pubmed/30516436 http://dx.doi.org/10.1091/mbc.E18-06-0401 Text en © 2019 Bonventre et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Brief Reports
Bonventre, Josephine A.
Holman, Chelsea
Manchanda, Aayushi
Codding, Sara J.
Chau, Trisha
Huegel, Jacob
Barton, Carrie
Tanguay, Robert
Johnson, Colin P.
Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title_full Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title_fullStr Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title_full_unstemmed Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title_short Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
title_sort fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589578/
https://www.ncbi.nlm.nih.gov/pubmed/30516436
http://dx.doi.org/10.1091/mbc.E18-06-0401
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