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salto/CG13164 is required for sperm head morphogenesis in Drosophila

Producing mature spermatozoa is essential for sexual reproduction in metazoans. Spermiogenesis involves dramatic cell morphological changes going from sperm tail elongation and nuclear reshaping to cell membrane remodeling during sperm individualization and release. The sperm manchette plays a criti...

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Autores principales: Augière, Céline, Lapart, Jean-André, Duteyrat, Jean-Luc, Cortier, Elisabeth, Maire, Charline, Thomas, Joëlle, Durand, Bénédicte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589691/
https://www.ncbi.nlm.nih.gov/pubmed/30601696
http://dx.doi.org/10.1091/mbc.E18-07-0429
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author Augière, Céline
Lapart, Jean-André
Duteyrat, Jean-Luc
Cortier, Elisabeth
Maire, Charline
Thomas, Joëlle
Durand, Bénédicte
author_facet Augière, Céline
Lapart, Jean-André
Duteyrat, Jean-Luc
Cortier, Elisabeth
Maire, Charline
Thomas, Joëlle
Durand, Bénédicte
author_sort Augière, Céline
collection PubMed
description Producing mature spermatozoa is essential for sexual reproduction in metazoans. Spermiogenesis involves dramatic cell morphological changes going from sperm tail elongation and nuclear reshaping to cell membrane remodeling during sperm individualization and release. The sperm manchette plays a critical scaffolding function during nuclear remodeling by linking the nuclear lamina to the cytoskeleton. Here, we describe the role of an uncharacterized protein in Drosophila, salto/CG13164, involved in nuclear shaping and spermatid individualization. Salto has dynamic localization during spermatid differentiation, being progressively relocated from the sperm-nuclear dense body, which is equivalent to the mammalian sperm manchette, to the centriolar adjunct and acrosomal cap during spermiogenesis. salto-null male flies are sterile and exhibit complete spermatid individualization defects. salto-deficient spermatids show coiled spermatid nuclei at late maturation stages and stalled individualization complexes. Our work sheds light on a novel component involved in cytoskeleton-based cell-morphological changes during spermiogenesis.
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spelling pubmed-65896912019-07-10 salto/CG13164 is required for sperm head morphogenesis in Drosophila Augière, Céline Lapart, Jean-André Duteyrat, Jean-Luc Cortier, Elisabeth Maire, Charline Thomas, Joëlle Durand, Bénédicte Mol Biol Cell Articles Producing mature spermatozoa is essential for sexual reproduction in metazoans. Spermiogenesis involves dramatic cell morphological changes going from sperm tail elongation and nuclear reshaping to cell membrane remodeling during sperm individualization and release. The sperm manchette plays a critical scaffolding function during nuclear remodeling by linking the nuclear lamina to the cytoskeleton. Here, we describe the role of an uncharacterized protein in Drosophila, salto/CG13164, involved in nuclear shaping and spermatid individualization. Salto has dynamic localization during spermatid differentiation, being progressively relocated from the sperm-nuclear dense body, which is equivalent to the mammalian sperm manchette, to the centriolar adjunct and acrosomal cap during spermiogenesis. salto-null male flies are sterile and exhibit complete spermatid individualization defects. salto-deficient spermatids show coiled spermatid nuclei at late maturation stages and stalled individualization complexes. Our work sheds light on a novel component involved in cytoskeleton-based cell-morphological changes during spermiogenesis. The American Society for Cell Biology 2019-03-01 /pmc/articles/PMC6589691/ /pubmed/30601696 http://dx.doi.org/10.1091/mbc.E18-07-0429 Text en © 2019 Augière et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Augière, Céline
Lapart, Jean-André
Duteyrat, Jean-Luc
Cortier, Elisabeth
Maire, Charline
Thomas, Joëlle
Durand, Bénédicte
salto/CG13164 is required for sperm head morphogenesis in Drosophila
title salto/CG13164 is required for sperm head morphogenesis in Drosophila
title_full salto/CG13164 is required for sperm head morphogenesis in Drosophila
title_fullStr salto/CG13164 is required for sperm head morphogenesis in Drosophila
title_full_unstemmed salto/CG13164 is required for sperm head morphogenesis in Drosophila
title_short salto/CG13164 is required for sperm head morphogenesis in Drosophila
title_sort salto/cg13164 is required for sperm head morphogenesis in drosophila
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589691/
https://www.ncbi.nlm.nih.gov/pubmed/30601696
http://dx.doi.org/10.1091/mbc.E18-07-0429
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