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NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling
BACKGROUND: NIMA related kinase 2 (NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers. However, the effect of the up-regulated NEK2 on cellular signaling in tumors, such as gastric cancer (GC), is con-fusing. AIM: To...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589739/ https://www.ncbi.nlm.nih.gov/pubmed/31249448 http://dx.doi.org/10.3748/wjg.v25.i23.2898 |
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author | Fan, Wei-Dong Chen, Tao Liu, Peng-Jun |
author_facet | Fan, Wei-Dong Chen, Tao Liu, Peng-Jun |
author_sort | Fan, Wei-Dong |
collection | PubMed |
description | BACKGROUND: NIMA related kinase 2 (NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers. However, the effect of the up-regulated NEK2 on cellular signaling in tumors, such as gastric cancer (GC), is con-fusing. AIM: To determine the role of the up-regulation of NEK2 in GC. METHODS: To investigate the pathological significance of NEK2 in GC, the expression pattern of NEK2 in GC was investigated based on the “Oncomain” database and compared between 30 pairs of cancer samples and adjacent tissues. The co-expression of NEK2 and ERK in GC was analyzed using The Cancer Genome Atlas (TCGA) database and confirmed in clinical samples by quantitative real-time PCR (qRT-PCR), and the survival curve was also plotted. Western blot or qRT-PCR was used to analyze the effect of NEK2 on the phosphorylation levels of ERK and c-JUN in two GC cell lines (BGC823 and SGC7901) with NEK2 overexpression, and the expression of the downstream effector cyclin D1. Furthermore, CCK8, EdU incorporation assay, and flow cytometry were used to detect the proliferative ability of BGC823 and SGC7901 cells with stably silenced ERK. RESULTS: NEK2 was significantly up-regulated in human GC tissues. ERK was significantly associated with NEK2 expression in human clinical specimens, and combined overexpression of NEK2 and ERK potentially forecasted a poor prognosis and survival in GC patients. NEK2 knockdown in GC cells inhibited ERK and c-JUN phosphory-lation and reduced the transcription of cyclin D1. More interestingly, NEK2 can rescue the inhibition of cellular viability, proliferation, and cell cycle progression due to ERK knockdown. CONCLUSION: Our results indicate that NEK2 plays a carcinogenic role in the malignant proliferation of GC cells via the ERK/MAPK signaling, which may be important for treatment and improving patient survival. |
format | Online Article Text |
id | pubmed-6589739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-65897392019-06-27 NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling Fan, Wei-Dong Chen, Tao Liu, Peng-Jun World J Gastroenterol Basic Study BACKGROUND: NIMA related kinase 2 (NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers. However, the effect of the up-regulated NEK2 on cellular signaling in tumors, such as gastric cancer (GC), is con-fusing. AIM: To determine the role of the up-regulation of NEK2 in GC. METHODS: To investigate the pathological significance of NEK2 in GC, the expression pattern of NEK2 in GC was investigated based on the “Oncomain” database and compared between 30 pairs of cancer samples and adjacent tissues. The co-expression of NEK2 and ERK in GC was analyzed using The Cancer Genome Atlas (TCGA) database and confirmed in clinical samples by quantitative real-time PCR (qRT-PCR), and the survival curve was also plotted. Western blot or qRT-PCR was used to analyze the effect of NEK2 on the phosphorylation levels of ERK and c-JUN in two GC cell lines (BGC823 and SGC7901) with NEK2 overexpression, and the expression of the downstream effector cyclin D1. Furthermore, CCK8, EdU incorporation assay, and flow cytometry were used to detect the proliferative ability of BGC823 and SGC7901 cells with stably silenced ERK. RESULTS: NEK2 was significantly up-regulated in human GC tissues. ERK was significantly associated with NEK2 expression in human clinical specimens, and combined overexpression of NEK2 and ERK potentially forecasted a poor prognosis and survival in GC patients. NEK2 knockdown in GC cells inhibited ERK and c-JUN phosphory-lation and reduced the transcription of cyclin D1. More interestingly, NEK2 can rescue the inhibition of cellular viability, proliferation, and cell cycle progression due to ERK knockdown. CONCLUSION: Our results indicate that NEK2 plays a carcinogenic role in the malignant proliferation of GC cells via the ERK/MAPK signaling, which may be important for treatment and improving patient survival. Baishideng Publishing Group Inc 2019-06-21 2019-06-21 /pmc/articles/PMC6589739/ /pubmed/31249448 http://dx.doi.org/10.3748/wjg.v25.i23.2898 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Fan, Wei-Dong Chen, Tao Liu, Peng-Jun NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title | NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title_full | NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title_fullStr | NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title_full_unstemmed | NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title_short | NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling |
title_sort | nima related kinase 2 promotes gastric cancer cell proliferation via erk/mapk signaling |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589739/ https://www.ncbi.nlm.nih.gov/pubmed/31249448 http://dx.doi.org/10.3748/wjg.v25.i23.2898 |
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