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GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin

The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of G...

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Autores principales: Ahat, Erpan, Xiang, Yi, Zhang, Xiaoyan, Bekier, Michael E., Wang, Yanzhuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589770/
https://www.ncbi.nlm.nih.gov/pubmed/30649990
http://dx.doi.org/10.1091/mbc.E18-07-0462
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author Ahat, Erpan
Xiang, Yi
Zhang, Xiaoyan
Bekier, Michael E.
Wang, Yanzhuang
author_facet Ahat, Erpan
Xiang, Yi
Zhang, Xiaoyan
Bekier, Michael E.
Wang, Yanzhuang
author_sort Ahat, Erpan
collection PubMed
description The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disrupts Golgi stack formation and impairs critical functions of the Golgi, such as accurate protein glycosylation and sorting. However, how Golgi destruction affects other cellular activities is so far unknown. Here, we report that depletion of GRASP proteins reduces cell attachment and migration. Interestingly, GRASP depletion reduces the protein level of α5β1 integrin, the major cell adhesion molecule at the surface of HeLa and MDA-MB-231 cells, due to decreased integrin protein synthesis. GRASP depletion also increases cell growth and total protein synthesis. These new findings enrich our understanding on the role of the Golgi in cell physiology and provide a potential target for treating protein-trafficking disorders.
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spelling pubmed-65897702019-07-15 GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin Ahat, Erpan Xiang, Yi Zhang, Xiaoyan Bekier, Michael E. Wang, Yanzhuang Mol Biol Cell Articles The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disrupts Golgi stack formation and impairs critical functions of the Golgi, such as accurate protein glycosylation and sorting. However, how Golgi destruction affects other cellular activities is so far unknown. Here, we report that depletion of GRASP proteins reduces cell attachment and migration. Interestingly, GRASP depletion reduces the protein level of α5β1 integrin, the major cell adhesion molecule at the surface of HeLa and MDA-MB-231 cells, due to decreased integrin protein synthesis. GRASP depletion also increases cell growth and total protein synthesis. These new findings enrich our understanding on the role of the Golgi in cell physiology and provide a potential target for treating protein-trafficking disorders. The American Society for Cell Biology 2019-03-15 /pmc/articles/PMC6589770/ /pubmed/30649990 http://dx.doi.org/10.1091/mbc.E18-07-0462 Text en © 2019 Ahat et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Ahat, Erpan
Xiang, Yi
Zhang, Xiaoyan
Bekier, Michael E.
Wang, Yanzhuang
GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title_full GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title_fullStr GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title_full_unstemmed GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title_short GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
title_sort grasp depletion–mediated golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589770/
https://www.ncbi.nlm.nih.gov/pubmed/30649990
http://dx.doi.org/10.1091/mbc.E18-07-0462
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