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A unified model for microtubule rescue

How microtubules transition from depolymerization to polymerization, known as rescue, is poorly understood. Here we examine two models for rescue: 1) an “end-driven” model in which the depolymerizing end stochastically switches to a stable state; and 2) a “lattice-driven” model in which rescue sites...

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Detalles Bibliográficos
Autores principales: Fees, Colby P., Moore, Jeffrey K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589779/
https://www.ncbi.nlm.nih.gov/pubmed/30672721
http://dx.doi.org/10.1091/mbc.E18-08-0541
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author Fees, Colby P.
Moore, Jeffrey K.
author_facet Fees, Colby P.
Moore, Jeffrey K.
author_sort Fees, Colby P.
collection PubMed
description How microtubules transition from depolymerization to polymerization, known as rescue, is poorly understood. Here we examine two models for rescue: 1) an “end-driven” model in which the depolymerizing end stochastically switches to a stable state; and 2) a “lattice-driven” model in which rescue sites are integrated into the microtubule before depolymerization. We test these models using a combination of computational simulations and in vitro experiments with purified tubulin. Our findings support the “lattice-driven” model by identifying repeated rescue sites in microtubules. In addition, we discover an important role for divalent cations in determining the frequency and location of rescue sites. We use “wash-in” experiments to show that divalent cations inhibit rescue during depolymerization, but not during polymerization. We propose a unified model in which rescues are driven by embedded rescue sites in microtubules, but the activity of these sites is influenced by changes in the depolymerizing ends.
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spelling pubmed-65897792019-07-15 A unified model for microtubule rescue Fees, Colby P. Moore, Jeffrey K. Mol Biol Cell Articles How microtubules transition from depolymerization to polymerization, known as rescue, is poorly understood. Here we examine two models for rescue: 1) an “end-driven” model in which the depolymerizing end stochastically switches to a stable state; and 2) a “lattice-driven” model in which rescue sites are integrated into the microtubule before depolymerization. We test these models using a combination of computational simulations and in vitro experiments with purified tubulin. Our findings support the “lattice-driven” model by identifying repeated rescue sites in microtubules. In addition, we discover an important role for divalent cations in determining the frequency and location of rescue sites. We use “wash-in” experiments to show that divalent cations inhibit rescue during depolymerization, but not during polymerization. We propose a unified model in which rescues are driven by embedded rescue sites in microtubules, but the activity of these sites is influenced by changes in the depolymerizing ends. The American Society for Cell Biology 2019-03-15 /pmc/articles/PMC6589779/ /pubmed/30672721 http://dx.doi.org/10.1091/mbc.E18-08-0541 Text en © 2019 Fees and Moore. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Fees, Colby P.
Moore, Jeffrey K.
A unified model for microtubule rescue
title A unified model for microtubule rescue
title_full A unified model for microtubule rescue
title_fullStr A unified model for microtubule rescue
title_full_unstemmed A unified model for microtubule rescue
title_short A unified model for microtubule rescue
title_sort unified model for microtubule rescue
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589779/
https://www.ncbi.nlm.nih.gov/pubmed/30672721
http://dx.doi.org/10.1091/mbc.E18-08-0541
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