Cargando…
Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. D...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590243/ https://www.ncbi.nlm.nih.gov/pubmed/30418682 http://dx.doi.org/10.1111/all.13663 |
_version_ | 1783429517277134848 |
---|---|
author | Boehm, Thomas Reiter, Birgit Ristl, Robin Petroczi, Karin Sperr, Wolfgang Stimpfl, Thomas Valent, Peter Jilma, Bernd |
author_facet | Boehm, Thomas Reiter, Birgit Ristl, Robin Petroczi, Karin Sperr, Wolfgang Stimpfl, Thomas Valent, Peter Jilma, Bernd |
author_sort | Boehm, Thomas |
collection | PubMed |
description | BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans. METHODS: Plasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations. RESULTS: Mean DAO (132 ng/mL) and tryptase (304 ng/mL) concentrations increased 187‐ and 4.0‐fold, respectively, over baseline values (DAO 0.7 ng/mL, tryptase 76 ng/mL) during severe anaphylaxis. Under non‐anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples. CONCLUSION: During severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin‐sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation. |
format | Online Article Text |
id | pubmed-6590243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65902432019-07-08 Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients Boehm, Thomas Reiter, Birgit Ristl, Robin Petroczi, Karin Sperr, Wolfgang Stimpfl, Thomas Valent, Peter Jilma, Bernd Allergy ORIGINAL ARTICLES BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans. METHODS: Plasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations. RESULTS: Mean DAO (132 ng/mL) and tryptase (304 ng/mL) concentrations increased 187‐ and 4.0‐fold, respectively, over baseline values (DAO 0.7 ng/mL, tryptase 76 ng/mL) during severe anaphylaxis. Under non‐anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples. CONCLUSION: During severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin‐sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation. John Wiley and Sons Inc. 2019-01-01 2019-03 /pmc/articles/PMC6590243/ /pubmed/30418682 http://dx.doi.org/10.1111/all.13663 Text en © 2018 The Authors. Allergy Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | ORIGINAL ARTICLES Boehm, Thomas Reiter, Birgit Ristl, Robin Petroczi, Karin Sperr, Wolfgang Stimpfl, Thomas Valent, Peter Jilma, Bernd Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title | Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title_full | Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title_fullStr | Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title_full_unstemmed | Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title_short | Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
title_sort | massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590243/ https://www.ncbi.nlm.nih.gov/pubmed/30418682 http://dx.doi.org/10.1111/all.13663 |
work_keys_str_mv | AT boehmthomas massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT reiterbirgit massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT ristlrobin massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT petroczikarin massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT sperrwolfgang massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT stimpflthomas massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT valentpeter massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients AT jilmabernd massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients |