Cargando…

Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients

BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. D...

Descripción completa

Detalles Bibliográficos
Autores principales: Boehm, Thomas, Reiter, Birgit, Ristl, Robin, Petroczi, Karin, Sperr, Wolfgang, Stimpfl, Thomas, Valent, Peter, Jilma, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590243/
https://www.ncbi.nlm.nih.gov/pubmed/30418682
http://dx.doi.org/10.1111/all.13663
_version_ 1783429517277134848
author Boehm, Thomas
Reiter, Birgit
Ristl, Robin
Petroczi, Karin
Sperr, Wolfgang
Stimpfl, Thomas
Valent, Peter
Jilma, Bernd
author_facet Boehm, Thomas
Reiter, Birgit
Ristl, Robin
Petroczi, Karin
Sperr, Wolfgang
Stimpfl, Thomas
Valent, Peter
Jilma, Bernd
author_sort Boehm, Thomas
collection PubMed
description BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans. METHODS: Plasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations. RESULTS: Mean DAO (132 ng/mL) and tryptase (304 ng/mL) concentrations increased 187‐ and 4.0‐fold, respectively, over baseline values (DAO 0.7 ng/mL, tryptase 76 ng/mL) during severe anaphylaxis. Under non‐anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples. CONCLUSION: During severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin‐sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation.
format Online
Article
Text
id pubmed-6590243
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-65902432019-07-08 Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients Boehm, Thomas Reiter, Birgit Ristl, Robin Petroczi, Karin Sperr, Wolfgang Stimpfl, Thomas Valent, Peter Jilma, Bernd Allergy ORIGINAL ARTICLES BACKGROUND: Histaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin‐sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans. METHODS: Plasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations. RESULTS: Mean DAO (132 ng/mL) and tryptase (304 ng/mL) concentrations increased 187‐ and 4.0‐fold, respectively, over baseline values (DAO 0.7 ng/mL, tryptase 76 ng/mL) during severe anaphylaxis. Under non‐anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples. CONCLUSION: During severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin‐sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation. John Wiley and Sons Inc. 2019-01-01 2019-03 /pmc/articles/PMC6590243/ /pubmed/30418682 http://dx.doi.org/10.1111/all.13663 Text en © 2018 The Authors. Allergy Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ORIGINAL ARTICLES
Boehm, Thomas
Reiter, Birgit
Ristl, Robin
Petroczi, Karin
Sperr, Wolfgang
Stimpfl, Thomas
Valent, Peter
Jilma, Bernd
Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title_full Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title_fullStr Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title_full_unstemmed Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title_short Massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
title_sort massive release of the histamine‐degrading enzyme diamine oxidase during severe anaphylaxis in mastocytosis patients
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590243/
https://www.ncbi.nlm.nih.gov/pubmed/30418682
http://dx.doi.org/10.1111/all.13663
work_keys_str_mv AT boehmthomas massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT reiterbirgit massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT ristlrobin massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT petroczikarin massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT sperrwolfgang massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT stimpflthomas massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT valentpeter massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients
AT jilmabernd massivereleaseofthehistaminedegradingenzymediamineoxidaseduringsevereanaphylaxisinmastocytosispatients