Cargando…
Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice
Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue a...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590305/ https://www.ncbi.nlm.nih.gov/pubmed/30656795 http://dx.doi.org/10.1111/asj.13160 |
_version_ | 1783429529600000000 |
---|---|
author | Kaneko, Hiroyuki Kikuchi, Kazuhiro Men, Nguyen Thi Noguchi, Junko |
author_facet | Kaneko, Hiroyuki Kikuchi, Kazuhiro Men, Nguyen Thi Noguchi, Junko |
author_sort | Kaneko, Hiroyuki |
collection | PubMed |
description | Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10‐, D30‐, and D60‐FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60‐FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH‐62.5 or ‐125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH‐125 group. The FSH‐125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice. |
format | Online Article Text |
id | pubmed-6590305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65903052019-07-08 Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice Kaneko, Hiroyuki Kikuchi, Kazuhiro Men, Nguyen Thi Noguchi, Junko Anim Sci J ORIGINAL ARTICLES Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10‐, D30‐, and D60‐FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60‐FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH‐62.5 or ‐125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH‐125 group. The FSH‐125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice. John Wiley and Sons Inc. 2019-01-17 2019-03 /pmc/articles/PMC6590305/ /pubmed/30656795 http://dx.doi.org/10.1111/asj.13160 Text en © 2019 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ORIGINAL ARTICLES Kaneko, Hiroyuki Kikuchi, Kazuhiro Men, Nguyen Thi Noguchi, Junko Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title | Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title_full | Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title_fullStr | Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title_full_unstemmed | Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title_short | Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice |
title_sort | developmental ability of oocytes retrieved from meishan neonatal ovarian tissue grafted into nude mice |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590305/ https://www.ncbi.nlm.nih.gov/pubmed/30656795 http://dx.doi.org/10.1111/asj.13160 |
work_keys_str_mv | AT kanekohiroyuki developmentalabilityofoocytesretrievedfrommeishanneonatalovariantissuegraftedintonudemice AT kikuchikazuhiro developmentalabilityofoocytesretrievedfrommeishanneonatalovariantissuegraftedintonudemice AT mennguyenthi developmentalabilityofoocytesretrievedfrommeishanneonatalovariantissuegraftedintonudemice AT noguchijunko developmentalabilityofoocytesretrievedfrommeishanneonatalovariantissuegraftedintonudemice |