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DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells

Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the ge...

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Detalles Bibliográficos
Autores principales: Di Trapani, Mariano, Manaresi, Nicolò, Medoro, Gianni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590341/
https://www.ncbi.nlm.nih.gov/pubmed/30551261
http://dx.doi.org/10.1002/cyto.a.23687
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author Di Trapani, Mariano
Manaresi, Nicolò
Medoro, Gianni
author_facet Di Trapani, Mariano
Manaresi, Nicolò
Medoro, Gianni
author_sort Di Trapani, Mariano
collection PubMed
description Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip‐based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image‐based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
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spelling pubmed-65903412019-07-08 DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells Di Trapani, Mariano Manaresi, Nicolò Medoro, Gianni Cytometry A Review Article Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip‐based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image‐based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. John Wiley & Sons, Inc. 2018-12-14 2018-12 /pmc/articles/PMC6590341/ /pubmed/30551261 http://dx.doi.org/10.1002/cyto.a.23687 Text en © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Review Article
Di Trapani, Mariano
Manaresi, Nicolò
Medoro, Gianni
DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title_full DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title_fullStr DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title_full_unstemmed DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title_short DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
title_sort deparray™ system: an automatic image‐based sorter for isolation of pure circulating tumor cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590341/
https://www.ncbi.nlm.nih.gov/pubmed/30551261
http://dx.doi.org/10.1002/cyto.a.23687
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