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DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the ge...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590341/ https://www.ncbi.nlm.nih.gov/pubmed/30551261 http://dx.doi.org/10.1002/cyto.a.23687 |
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author | Di Trapani, Mariano Manaresi, Nicolò Medoro, Gianni |
author_facet | Di Trapani, Mariano Manaresi, Nicolò Medoro, Gianni |
author_sort | Di Trapani, Mariano |
collection | PubMed |
description | Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip‐based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image‐based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. |
format | Online Article Text |
id | pubmed-6590341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65903412019-07-08 DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells Di Trapani, Mariano Manaresi, Nicolò Medoro, Gianni Cytometry A Review Article Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip‐based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image‐based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. John Wiley & Sons, Inc. 2018-12-14 2018-12 /pmc/articles/PMC6590341/ /pubmed/30551261 http://dx.doi.org/10.1002/cyto.a.23687 Text en © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Review Article Di Trapani, Mariano Manaresi, Nicolò Medoro, Gianni DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title | DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title_full | DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title_fullStr | DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title_full_unstemmed | DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title_short | DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells |
title_sort | deparray™ system: an automatic image‐based sorter for isolation of pure circulating tumor cells |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590341/ https://www.ncbi.nlm.nih.gov/pubmed/30551261 http://dx.doi.org/10.1002/cyto.a.23687 |
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