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High Expression and Clinical Significance of Elafin in Colorectal Cancer
OBJECTIVE: The decrease of Elafin is associated with several inflammatory diseases. Exogenous Elafin may be a treatment for IBD. Little data has shown the expression of Elafin in patients of colorectal cancer. Here, we tried to explore Elafin expression in human tissues of colorectal cancer. METHODS...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590570/ https://www.ncbi.nlm.nih.gov/pubmed/31281349 http://dx.doi.org/10.1155/2019/4946824 |
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author | Liu, Yun Tian, Yu Wu, Ting Dai, Yun Wang, Weihong Teng, Guigen |
author_facet | Liu, Yun Tian, Yu Wu, Ting Dai, Yun Wang, Weihong Teng, Guigen |
author_sort | Liu, Yun |
collection | PubMed |
description | OBJECTIVE: The decrease of Elafin is associated with several inflammatory diseases. Exogenous Elafin may be a treatment for IBD. Little data has shown the expression of Elafin in patients of colorectal cancer. Here, we tried to explore Elafin expression in human tissues of colorectal cancer. METHODS: We examined the protein expression of Elafin in human tissues of adjacent nontumor and colorectal tumor by immunohistochemistry (IHC) or quantitative real-time polymerase chain reaction (qRT-PCR), then analyzed the clinical and RNA-seq data presented in The Cancer Genome Atlas (TCGA) database to confirm the relationship between Elafin levels and colorectal tumor. RESULTS: Of the 88 paired samples, 68 colorectal cancer tissues indicated a high expression of Elafin compared with 52 matched adjacent noncancerous tissues. And the mRNA levels of Elafin in 35 paired tissues showed a similar trend. The RNA-seq and clinical data were available in 438 colorectal cancer tissues and 41 normal tissues in TCGA database. The RNA-seq data showed that Elafin mRNA was upregulated about twofold in colorectal cancer samples as compared to adjacent noncancerous samples (176.42 ± 402.13 vs. 96.75 ± 150.07; P = 0.208). No statistically significant correlation was found between the Elafin expression and the age, gender, tumor invasive stage, lymph node metastasis, and distant metastasis both at the protein and mRNA levels. However, the Elafin expression was correlated with clinical stage based on the AJCC guidelines at protein levels but not mRNA levels. CONCLUSIONS: Elafin was upregulated in patients of colorectal cancer, resulting to potential limitations for exogenous Elafin treatment. |
format | Online Article Text |
id | pubmed-6590570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-65905702019-07-07 High Expression and Clinical Significance of Elafin in Colorectal Cancer Liu, Yun Tian, Yu Wu, Ting Dai, Yun Wang, Weihong Teng, Guigen Gastroenterol Res Pract Research Article OBJECTIVE: The decrease of Elafin is associated with several inflammatory diseases. Exogenous Elafin may be a treatment for IBD. Little data has shown the expression of Elafin in patients of colorectal cancer. Here, we tried to explore Elafin expression in human tissues of colorectal cancer. METHODS: We examined the protein expression of Elafin in human tissues of adjacent nontumor and colorectal tumor by immunohistochemistry (IHC) or quantitative real-time polymerase chain reaction (qRT-PCR), then analyzed the clinical and RNA-seq data presented in The Cancer Genome Atlas (TCGA) database to confirm the relationship between Elafin levels and colorectal tumor. RESULTS: Of the 88 paired samples, 68 colorectal cancer tissues indicated a high expression of Elafin compared with 52 matched adjacent noncancerous tissues. And the mRNA levels of Elafin in 35 paired tissues showed a similar trend. The RNA-seq and clinical data were available in 438 colorectal cancer tissues and 41 normal tissues in TCGA database. The RNA-seq data showed that Elafin mRNA was upregulated about twofold in colorectal cancer samples as compared to adjacent noncancerous samples (176.42 ± 402.13 vs. 96.75 ± 150.07; P = 0.208). No statistically significant correlation was found between the Elafin expression and the age, gender, tumor invasive stage, lymph node metastasis, and distant metastasis both at the protein and mRNA levels. However, the Elafin expression was correlated with clinical stage based on the AJCC guidelines at protein levels but not mRNA levels. CONCLUSIONS: Elafin was upregulated in patients of colorectal cancer, resulting to potential limitations for exogenous Elafin treatment. Hindawi 2019-06-09 /pmc/articles/PMC6590570/ /pubmed/31281349 http://dx.doi.org/10.1155/2019/4946824 Text en Copyright © 2019 Yun Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Yun Tian, Yu Wu, Ting Dai, Yun Wang, Weihong Teng, Guigen High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title | High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title_full | High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title_fullStr | High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title_full_unstemmed | High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title_short | High Expression and Clinical Significance of Elafin in Colorectal Cancer |
title_sort | high expression and clinical significance of elafin in colorectal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590570/ https://www.ncbi.nlm.nih.gov/pubmed/31281349 http://dx.doi.org/10.1155/2019/4946824 |
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