A simplified workflow for monoclonal antibody sequencing

The diversity of antibody variable regions makes cDNA sequencing challenging, and conventional monoclonal antibody cDNA amplification requires the use of degenerate primers. Here, we describe a simplified workflow for amplification of IgG antibody variable regions from hybridoma RNA by a specialized...

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Detalles Bibliográficos
Autores principales: Meyer, Lena, López, Tomás, Espinosa, Rafaela, Arias, Carlos F., Vollmers, Christopher, DuBois, Rebecca M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590890/
https://www.ncbi.nlm.nih.gov/pubmed/31233538
http://dx.doi.org/10.1371/journal.pone.0218717

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590890/
https://www.ncbi.nlm.nih.gov/pubmed/31233538
http://dx.doi.org/10.1371/journal.pone.0218717

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