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Connexin-36 distribution and layer-specific topography in the cat retina
Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calreti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591202/ https://www.ncbi.nlm.nih.gov/pubmed/31172263 http://dx.doi.org/10.1007/s00429-019-01876-y |
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author | Telkes, Ildikó Kóbor, Péter Orbán, József Kovács-Öller, Tamás Völgyi, Béla Buzás, Péter |
author_facet | Telkes, Ildikó Kóbor, Péter Orbán, József Kovács-Öller, Tamás Völgyi, Béla Buzás, Péter |
author_sort | Telkes, Ildikó |
collection | PubMed |
description | Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calretinin-immunoreactive (CaR-IR) cell populations served to outline the nuclear and plexiform layers and to stain specific neuronal populations. CaR-IR cells included horizontal cells in the outer retina, numerous amacrine cells, and scattered cells in the ganglion cell layer. Cx36-IR plaques were found among horizontal cell dendrites albeit without systematic colocalization of the two labels. Diffuse Cx36 immunoreactivity was found in the cytoplasm of AII amacrine cells, but no colocalization of Cx36 plaques was observed with either the perikarya or the long varicose dendrites of the CaR-IR non-AII amacrine cells. Cx36 puncta were seen throughout the entire inner plexiform layer showing their highest density in the ON sublamina. The densities of AII amacrine cell bodies and Cx36 plaques in the ON sublamina were strongly correlated across a wide range of eccentricities suggesting their anatomical association. However, the high number of plaques per AII cell suggests that a considerable fraction of Cx36 gap junctions in the ON sublamina is formed by other cell types than AII amacrine cells drawing attention to extensive but less studied electrically coupled networks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01876-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6591202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-65912022019-07-11 Connexin-36 distribution and layer-specific topography in the cat retina Telkes, Ildikó Kóbor, Péter Orbán, József Kovács-Öller, Tamás Völgyi, Béla Buzás, Péter Brain Struct Funct Original Article Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calretinin-immunoreactive (CaR-IR) cell populations served to outline the nuclear and plexiform layers and to stain specific neuronal populations. CaR-IR cells included horizontal cells in the outer retina, numerous amacrine cells, and scattered cells in the ganglion cell layer. Cx36-IR plaques were found among horizontal cell dendrites albeit without systematic colocalization of the two labels. Diffuse Cx36 immunoreactivity was found in the cytoplasm of AII amacrine cells, but no colocalization of Cx36 plaques was observed with either the perikarya or the long varicose dendrites of the CaR-IR non-AII amacrine cells. Cx36 puncta were seen throughout the entire inner plexiform layer showing their highest density in the ON sublamina. The densities of AII amacrine cell bodies and Cx36 plaques in the ON sublamina were strongly correlated across a wide range of eccentricities suggesting their anatomical association. However, the high number of plaques per AII cell suggests that a considerable fraction of Cx36 gap junctions in the ON sublamina is formed by other cell types than AII amacrine cells drawing attention to extensive but less studied electrically coupled networks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01876-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-06-06 2019 /pmc/articles/PMC6591202/ /pubmed/31172263 http://dx.doi.org/10.1007/s00429-019-01876-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Telkes, Ildikó Kóbor, Péter Orbán, József Kovács-Öller, Tamás Völgyi, Béla Buzás, Péter Connexin-36 distribution and layer-specific topography in the cat retina |
title | Connexin-36 distribution and layer-specific topography in the cat retina |
title_full | Connexin-36 distribution and layer-specific topography in the cat retina |
title_fullStr | Connexin-36 distribution and layer-specific topography in the cat retina |
title_full_unstemmed | Connexin-36 distribution and layer-specific topography in the cat retina |
title_short | Connexin-36 distribution and layer-specific topography in the cat retina |
title_sort | connexin-36 distribution and layer-specific topography in the cat retina |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591202/ https://www.ncbi.nlm.nih.gov/pubmed/31172263 http://dx.doi.org/10.1007/s00429-019-01876-y |
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