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Connexin-36 distribution and layer-specific topography in the cat retina

Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calreti...

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Autores principales: Telkes, Ildikó, Kóbor, Péter, Orbán, József, Kovács-Öller, Tamás, Völgyi, Béla, Buzás, Péter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591202/
https://www.ncbi.nlm.nih.gov/pubmed/31172263
http://dx.doi.org/10.1007/s00429-019-01876-y
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author Telkes, Ildikó
Kóbor, Péter
Orbán, József
Kovács-Öller, Tamás
Völgyi, Béla
Buzás, Péter
author_facet Telkes, Ildikó
Kóbor, Péter
Orbán, József
Kovács-Öller, Tamás
Völgyi, Béla
Buzás, Péter
author_sort Telkes, Ildikó
collection PubMed
description Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calretinin-immunoreactive (CaR-IR) cell populations served to outline the nuclear and plexiform layers and to stain specific neuronal populations. CaR-IR cells included horizontal cells in the outer retina, numerous amacrine cells, and scattered cells in the ganglion cell layer. Cx36-IR plaques were found among horizontal cell dendrites albeit without systematic colocalization of the two labels. Diffuse Cx36 immunoreactivity was found in the cytoplasm of AII amacrine cells, but no colocalization of Cx36 plaques was observed with either the perikarya or the long varicose dendrites of the CaR-IR non-AII amacrine cells. Cx36 puncta were seen throughout the entire inner plexiform layer showing their highest density in the ON sublamina. The densities of AII amacrine cell bodies and Cx36 plaques in the ON sublamina were strongly correlated across a wide range of eccentricities suggesting their anatomical association. However, the high number of plaques per AII cell suggests that a considerable fraction of Cx36 gap junctions in the ON sublamina is formed by other cell types than AII amacrine cells drawing attention to extensive but less studied electrically coupled networks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01876-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-65912022019-07-11 Connexin-36 distribution and layer-specific topography in the cat retina Telkes, Ildikó Kóbor, Péter Orbán, József Kovács-Öller, Tamás Völgyi, Béla Buzás, Péter Brain Struct Funct Original Article Connexin-36 (Cx36) is the major constituent of mammalian retinal gap junctions positioned in key signal pathways. Here, we examined the laminar and large-scale topographical distribution of Cx36 punctate immunolabels in the retina of the cat, a classical model of the mammalian visual system. Calretinin-immunoreactive (CaR-IR) cell populations served to outline the nuclear and plexiform layers and to stain specific neuronal populations. CaR-IR cells included horizontal cells in the outer retina, numerous amacrine cells, and scattered cells in the ganglion cell layer. Cx36-IR plaques were found among horizontal cell dendrites albeit without systematic colocalization of the two labels. Diffuse Cx36 immunoreactivity was found in the cytoplasm of AII amacrine cells, but no colocalization of Cx36 plaques was observed with either the perikarya or the long varicose dendrites of the CaR-IR non-AII amacrine cells. Cx36 puncta were seen throughout the entire inner plexiform layer showing their highest density in the ON sublamina. The densities of AII amacrine cell bodies and Cx36 plaques in the ON sublamina were strongly correlated across a wide range of eccentricities suggesting their anatomical association. However, the high number of plaques per AII cell suggests that a considerable fraction of Cx36 gap junctions in the ON sublamina is formed by other cell types than AII amacrine cells drawing attention to extensive but less studied electrically coupled networks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01876-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-06-06 2019 /pmc/articles/PMC6591202/ /pubmed/31172263 http://dx.doi.org/10.1007/s00429-019-01876-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Telkes, Ildikó
Kóbor, Péter
Orbán, József
Kovács-Öller, Tamás
Völgyi, Béla
Buzás, Péter
Connexin-36 distribution and layer-specific topography in the cat retina
title Connexin-36 distribution and layer-specific topography in the cat retina
title_full Connexin-36 distribution and layer-specific topography in the cat retina
title_fullStr Connexin-36 distribution and layer-specific topography in the cat retina
title_full_unstemmed Connexin-36 distribution and layer-specific topography in the cat retina
title_short Connexin-36 distribution and layer-specific topography in the cat retina
title_sort connexin-36 distribution and layer-specific topography in the cat retina
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591202/
https://www.ncbi.nlm.nih.gov/pubmed/31172263
http://dx.doi.org/10.1007/s00429-019-01876-y
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