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Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions

Diverse antibody repertoires are generated through remote genomic interactions involving immunoglobulin variable (V(H)), diversity (D(H)) and joining (J(H)) gene segments. How such interactions are orchestrated remains unknown. Here we develop a strategy to track V(H)-D(H)J(H) motion in B-lymphocyte...

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Autores principales: Khanna, Nimish, Zhang, Yaojun, Lucas, Joseph S., Dudko, Olga K., Murre, Cornelis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591236/
https://www.ncbi.nlm.nih.gov/pubmed/31235807
http://dx.doi.org/10.1038/s41467-019-10628-9
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author Khanna, Nimish
Zhang, Yaojun
Lucas, Joseph S.
Dudko, Olga K.
Murre, Cornelis
author_facet Khanna, Nimish
Zhang, Yaojun
Lucas, Joseph S.
Dudko, Olga K.
Murre, Cornelis
author_sort Khanna, Nimish
collection PubMed
description Diverse antibody repertoires are generated through remote genomic interactions involving immunoglobulin variable (V(H)), diversity (D(H)) and joining (J(H)) gene segments. How such interactions are orchestrated remains unknown. Here we develop a strategy to track V(H)-D(H)J(H) motion in B-lymphocytes. We find that V(H) and D(H)J(H) segments are trapped in configurations that allow only local motion, such that spatially proximal segments remain in proximity, while spatially remote segments remain remote. Within a subset of cells, however, abrupt changes in V(H)-D(H)J(H) motion are observed, plausibly caused by temporal alterations in chromatin configurations. Comparison of experimental and simulated data suggests that constrained motion is imposed by a network of cross-linked chromatin chains characteristic of a gel phase, yet poised near the sol phase, a solution of independent chromatin chains. These results suggest that chromosome organization near the sol-gel phase transition dictates the timing of genomic interactions to orchestrate gene expression and somatic recombination.
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spelling pubmed-65912362019-06-26 Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions Khanna, Nimish Zhang, Yaojun Lucas, Joseph S. Dudko, Olga K. Murre, Cornelis Nat Commun Article Diverse antibody repertoires are generated through remote genomic interactions involving immunoglobulin variable (V(H)), diversity (D(H)) and joining (J(H)) gene segments. How such interactions are orchestrated remains unknown. Here we develop a strategy to track V(H)-D(H)J(H) motion in B-lymphocytes. We find that V(H) and D(H)J(H) segments are trapped in configurations that allow only local motion, such that spatially proximal segments remain in proximity, while spatially remote segments remain remote. Within a subset of cells, however, abrupt changes in V(H)-D(H)J(H) motion are observed, plausibly caused by temporal alterations in chromatin configurations. Comparison of experimental and simulated data suggests that constrained motion is imposed by a network of cross-linked chromatin chains characteristic of a gel phase, yet poised near the sol phase, a solution of independent chromatin chains. These results suggest that chromosome organization near the sol-gel phase transition dictates the timing of genomic interactions to orchestrate gene expression and somatic recombination. Nature Publishing Group UK 2019-06-24 /pmc/articles/PMC6591236/ /pubmed/31235807 http://dx.doi.org/10.1038/s41467-019-10628-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Khanna, Nimish
Zhang, Yaojun
Lucas, Joseph S.
Dudko, Olga K.
Murre, Cornelis
Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title_full Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title_fullStr Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title_full_unstemmed Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title_short Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
title_sort chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591236/
https://www.ncbi.nlm.nih.gov/pubmed/31235807
http://dx.doi.org/10.1038/s41467-019-10628-9
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