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The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway

As a typical alpine wetland on the Tibetan Plateau, the Zoige wetland processes a large carbon stock and is a hotspot of methane emission. To date, many studies have investigated the methane flux in this wetland; however, the research on the source of methane in the soils of Zoige wetland is not cle...

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Autores principales: Zhang, Yanfen, Ma, Anzhou, Zhuang, Guoqiang, Zhuang, Xuliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591398/
https://www.ncbi.nlm.nih.gov/pubmed/31235734
http://dx.doi.org/10.1038/s41598-019-45590-5
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author Zhang, Yanfen
Ma, Anzhou
Zhuang, Guoqiang
Zhuang, Xuliang
author_facet Zhang, Yanfen
Ma, Anzhou
Zhuang, Guoqiang
Zhuang, Xuliang
author_sort Zhang, Yanfen
collection PubMed
description As a typical alpine wetland on the Tibetan Plateau, the Zoige wetland processes a large carbon stock and is a hotspot of methane emission. To date, many studies have investigated the methane flux in this wetland; however, the research on the source of methane in the soils of Zoige wetland is not clear enough. In this study, we determined the dynamic characteristics of the stable carbon isotopes during the methanogenesis of Zoige wetland soil and the corresponding microbial changes. The results showed that the δ(13)CH(4) varied between −19.86‰ and −28.32‰ and the α(C) ranged from 1.0029 to 1.0104 in the methanogenesis process, which suggests the dominance of acetotrophic methanogenesis. And among the increased methanogens, acetotrophic methanogens multiplied more obviously than hydrogenotrophic menthanogens. In addition, the results of structural equation models showed that the variations in stable carbon isotopes during the process were mainly affected by acetotrophic methanogens. Although the acetotrophic pathway was dominate, the varied isotope characteristics, increased methanogens and ratio of carbon dioxide to methane all showed that hydrogenotrophic and acetotrophic methanogenesis coexisted in the Zoige wetland. Overall, our study provided a detailed and definitive information to the source of methane in the soil of the Zoige wetland and laid a foundation of mechanism to the research of greenhouse gas in this alpine wetland.
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spelling pubmed-65913982019-07-02 The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway Zhang, Yanfen Ma, Anzhou Zhuang, Guoqiang Zhuang, Xuliang Sci Rep Article As a typical alpine wetland on the Tibetan Plateau, the Zoige wetland processes a large carbon stock and is a hotspot of methane emission. To date, many studies have investigated the methane flux in this wetland; however, the research on the source of methane in the soils of Zoige wetland is not clear enough. In this study, we determined the dynamic characteristics of the stable carbon isotopes during the methanogenesis of Zoige wetland soil and the corresponding microbial changes. The results showed that the δ(13)CH(4) varied between −19.86‰ and −28.32‰ and the α(C) ranged from 1.0029 to 1.0104 in the methanogenesis process, which suggests the dominance of acetotrophic methanogenesis. And among the increased methanogens, acetotrophic methanogens multiplied more obviously than hydrogenotrophic menthanogens. In addition, the results of structural equation models showed that the variations in stable carbon isotopes during the process were mainly affected by acetotrophic methanogens. Although the acetotrophic pathway was dominate, the varied isotope characteristics, increased methanogens and ratio of carbon dioxide to methane all showed that hydrogenotrophic and acetotrophic methanogenesis coexisted in the Zoige wetland. Overall, our study provided a detailed and definitive information to the source of methane in the soil of the Zoige wetland and laid a foundation of mechanism to the research of greenhouse gas in this alpine wetland. Nature Publishing Group UK 2019-06-24 /pmc/articles/PMC6591398/ /pubmed/31235734 http://dx.doi.org/10.1038/s41598-019-45590-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Yanfen
Ma, Anzhou
Zhuang, Guoqiang
Zhuang, Xuliang
The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title_full The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title_fullStr The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title_full_unstemmed The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title_short The acetotrophic pathway dominates methane production in Zoige alpine wetland coexisting with hydrogenotrophic pathway
title_sort acetotrophic pathway dominates methane production in zoige alpine wetland coexisting with hydrogenotrophic pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591398/
https://www.ncbi.nlm.nih.gov/pubmed/31235734
http://dx.doi.org/10.1038/s41598-019-45590-5
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