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Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human
Spermatogenesis is a complicated process including spermatogonial stem cells self-renewal and differentiates into mature spermatozoa. MicroRNAs (miRNAs) as a class of small non-coding RNAs play a crucial role during the process of spermatogenesis. However, the function of a plenty of miRNAs on sperm...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591449/ https://www.ncbi.nlm.nih.gov/pubmed/31275170 http://dx.doi.org/10.3389/fphys.2019.00765 |
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author | Gao, Huihui Wen, Hui Cao, Congcong Dong, Daqian Yang, Chenhao Xie, Shengsong Zhang, Jin Huang, Xunbin Huang, Xingxu Yuan, Shuiqiao Dong, Wuzi |
author_facet | Gao, Huihui Wen, Hui Cao, Congcong Dong, Daqian Yang, Chenhao Xie, Shengsong Zhang, Jin Huang, Xunbin Huang, Xingxu Yuan, Shuiqiao Dong, Wuzi |
author_sort | Gao, Huihui |
collection | PubMed |
description | Spermatogenesis is a complicated process including spermatogonial stem cells self-renewal and differentiates into mature spermatozoa. MicroRNAs (miRNAs) as a class of small non-coding RNAs play a crucial role during the process of spermatogenesis. However, the function of a plenty of miRNAs on spermatogenesis and the potential mechanisms remain largely unknown. Here, we show that genetically conditional overexpressed miR-10a in germ cells caused complete male sterility, characterized by meiotic arrested in germ cells. Analysis of miR-10a overexpression mouse testes reveals that failure of double strand break (DSB) repairs and aberrant spermatogonial differentiation. Furthermore, we identified Rad51 as a key target of miR-10a in germ cell by bioinformatics prediction and luciferase assay, which may be responsible for the infertility of the miR-10a overexpressed mice and germ cell arrested patients. Our data show that miR-10a dependent genetic regulation of meiotic process is crucial for male germ cell development and spermatogenesis in both mouse and human. These findings facilitate our understanding of the roles of miRNA-10a in spermatogenesis and male fertility. |
format | Online Article Text |
id | pubmed-6591449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65914492019-07-02 Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human Gao, Huihui Wen, Hui Cao, Congcong Dong, Daqian Yang, Chenhao Xie, Shengsong Zhang, Jin Huang, Xunbin Huang, Xingxu Yuan, Shuiqiao Dong, Wuzi Front Physiol Physiology Spermatogenesis is a complicated process including spermatogonial stem cells self-renewal and differentiates into mature spermatozoa. MicroRNAs (miRNAs) as a class of small non-coding RNAs play a crucial role during the process of spermatogenesis. However, the function of a plenty of miRNAs on spermatogenesis and the potential mechanisms remain largely unknown. Here, we show that genetically conditional overexpressed miR-10a in germ cells caused complete male sterility, characterized by meiotic arrested in germ cells. Analysis of miR-10a overexpression mouse testes reveals that failure of double strand break (DSB) repairs and aberrant spermatogonial differentiation. Furthermore, we identified Rad51 as a key target of miR-10a in germ cell by bioinformatics prediction and luciferase assay, which may be responsible for the infertility of the miR-10a overexpressed mice and germ cell arrested patients. Our data show that miR-10a dependent genetic regulation of meiotic process is crucial for male germ cell development and spermatogenesis in both mouse and human. These findings facilitate our understanding of the roles of miRNA-10a in spermatogenesis and male fertility. Frontiers Media S.A. 2019-06-18 /pmc/articles/PMC6591449/ /pubmed/31275170 http://dx.doi.org/10.3389/fphys.2019.00765 Text en Copyright © 2019 Gao, Wen, Cao, Dong, Yang, Xie, Zhang, Huang, Huang, Yuan and Dong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Gao, Huihui Wen, Hui Cao, Congcong Dong, Daqian Yang, Chenhao Xie, Shengsong Zhang, Jin Huang, Xunbin Huang, Xingxu Yuan, Shuiqiao Dong, Wuzi Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title | Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title_full | Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title_fullStr | Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title_full_unstemmed | Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title_short | Overexpression of MicroRNA-10a in Germ Cells Causes Male Infertility by Targeting Rad51 in Mouse and Human |
title_sort | overexpression of microrna-10a in germ cells causes male infertility by targeting rad51 in mouse and human |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591449/ https://www.ncbi.nlm.nih.gov/pubmed/31275170 http://dx.doi.org/10.3389/fphys.2019.00765 |
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