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Low-diluted Phenacetinum disrupted the melanoma cancer cell migration
Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591484/ https://www.ncbi.nlm.nih.gov/pubmed/31235855 http://dx.doi.org/10.1038/s41598-019-45578-1 |
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author | Fuselier, Camille Terryn, Christine Berquand, Alexandre Crowet, Jean-Marc Bonnomet, Arnaud Molinari, Michael Dauchez, Manuel Martiny, Laurent Schneider, Christophe |
author_facet | Fuselier, Camille Terryn, Christine Berquand, Alexandre Crowet, Jean-Marc Bonnomet, Arnaud Molinari, Michael Dauchez, Manuel Martiny, Laurent Schneider, Christophe |
author_sort | Fuselier, Camille |
collection | PubMed |
description | Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane protrusions, allow tumor cells to move along a determined pathway. Our study analyzed the mechanism of action of low-diluted Phenacetinum on murine cutaneous melanoma process in a fibronectin matrix environment. We demonstrated a reduction of dispersed cell migration, early and for as long as 24 h, by altering the formation of cell protrusions. Moreover, low-diluted Phenacetinum decreased cell stiffness highly on peripheral areas, due to a disruption of actin filaments located just under the plasma membrane. Finally, it modified the structure of the plasma membrane by accumulating large ordered lipid domains and disrupted B16 cell migration by a likely shift in the balance between ordered and disordered lipid phases. Whereas the correlation between the excess of lipid raft and cytoskeleton disrupting is not as yet established, it is clear that low-diluted Phenacetinum acts on the actin cytoskeleton organization, as confirmed by a decrease of cell stiffness affecting ultimately the establishment of an effective migration process. |
format | Online Article Text |
id | pubmed-6591484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65914842019-07-03 Low-diluted Phenacetinum disrupted the melanoma cancer cell migration Fuselier, Camille Terryn, Christine Berquand, Alexandre Crowet, Jean-Marc Bonnomet, Arnaud Molinari, Michael Dauchez, Manuel Martiny, Laurent Schneider, Christophe Sci Rep Article Dynamic and reciprocal interactions generated by the communication between tumor cells and their matrix microenvironment, play a major role in the progression of a tumor. Indeed, the adhesion of specific sites to matrix components, associated with the repeated and coordinated formation of membrane protrusions, allow tumor cells to move along a determined pathway. Our study analyzed the mechanism of action of low-diluted Phenacetinum on murine cutaneous melanoma process in a fibronectin matrix environment. We demonstrated a reduction of dispersed cell migration, early and for as long as 24 h, by altering the formation of cell protrusions. Moreover, low-diluted Phenacetinum decreased cell stiffness highly on peripheral areas, due to a disruption of actin filaments located just under the plasma membrane. Finally, it modified the structure of the plasma membrane by accumulating large ordered lipid domains and disrupted B16 cell migration by a likely shift in the balance between ordered and disordered lipid phases. Whereas the correlation between the excess of lipid raft and cytoskeleton disrupting is not as yet established, it is clear that low-diluted Phenacetinum acts on the actin cytoskeleton organization, as confirmed by a decrease of cell stiffness affecting ultimately the establishment of an effective migration process. Nature Publishing Group UK 2019-06-24 /pmc/articles/PMC6591484/ /pubmed/31235855 http://dx.doi.org/10.1038/s41598-019-45578-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fuselier, Camille Terryn, Christine Berquand, Alexandre Crowet, Jean-Marc Bonnomet, Arnaud Molinari, Michael Dauchez, Manuel Martiny, Laurent Schneider, Christophe Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title | Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title_full | Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title_fullStr | Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title_full_unstemmed | Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title_short | Low-diluted Phenacetinum disrupted the melanoma cancer cell migration |
title_sort | low-diluted phenacetinum disrupted the melanoma cancer cell migration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591484/ https://www.ncbi.nlm.nih.gov/pubmed/31235855 http://dx.doi.org/10.1038/s41598-019-45578-1 |
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