A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count

Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perfor...

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Detalles Bibliográficos
Autores principales: GRECU, AF, GRECU, DC, NICA, O, CIUCA, EM, CAMEN, A, CIUREA, ME
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medical University Publishing House Craiova 2019
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592675/
https://www.ncbi.nlm.nih.gov/pubmed/31297270
http://dx.doi.org/10.12865/CHSJ.45.01.14
Descripción
Sumario:Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. Materials and Methods: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. Results: PRF samples analysis showed persistent results pertaining to known PRF composition. Conclusions: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.

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