A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count

Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perfor...

Descripción completa

Detalles Bibliográficos
Autores principales: GRECU, AF, GRECU, DC, NICA, O, CIUCA, EM, CAMEN, A, CIUREA, ME
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medical University Publishing House Craiova 2019
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592675/
https://www.ncbi.nlm.nih.gov/pubmed/31297270
http://dx.doi.org/10.12865/CHSJ.45.01.14
_version_ 1783429921663614976
author GRECU, AF
GRECU, DC
NICA, O
CIUCA, EM
CAMEN, A
CIUREA, ME
author_facet GRECU, AF
GRECU, DC
NICA, O
CIUCA, EM
CAMEN, A
CIUREA, ME
author_sort GRECU, AF
collection PubMed
description Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. Materials and Methods: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. Results: PRF samples analysis showed persistent results pertaining to known PRF composition. Conclusions: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.
format Online
Article
Text
id pubmed-6592675
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Medical University Publishing House Craiova
record_format MEDLINE/PubMed
spelling pubmed-65926752019-07-11 A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count GRECU, AF GRECU, DC NICA, O CIUCA, EM CAMEN, A CIUREA, ME Curr Health Sci J Original Paper Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. Materials and Methods: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. Results: PRF samples analysis showed persistent results pertaining to known PRF composition. Conclusions: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats. Medical University Publishing House Craiova 2019 2019-03-31 /pmc/articles/PMC6592675/ /pubmed/31297270 http://dx.doi.org/10.12865/CHSJ.45.01.14 Text en Copyright © 2019, Medical University Publishing House Craiova http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited.
spellingShingle Original Paper
GRECU, AF
GRECU, DC
NICA, O
CIUCA, EM
CAMEN, A
CIUREA, ME
A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title_full A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title_fullStr A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title_full_unstemmed A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title_short A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count
title_sort novel method of obtaining platelet rich fibrin from rats and quantifying platelet count
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592675/
https://www.ncbi.nlm.nih.gov/pubmed/31297270
http://dx.doi.org/10.12865/CHSJ.45.01.14
work_keys_str_mv AT grecuaf anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT grecudc anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT nicao anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT ciucaem anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT camena anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT ciureame anovelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT grecuaf novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT grecudc novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT nicao novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT ciucaem novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT camena novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount
AT ciureame novelmethodofobtainingplateletrichfibrinfromratsandquantifyingplateletcount

Ejemplares similares