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Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis
The control of cell fate through oriented cell division is imperative for proper organ development. Basal epidermal progenitor cells divide parallel or perpendicular to the basement membrane to self-renew or produce differentiated stratified layers, but the mechanisms regulating the choice between d...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592681/ https://www.ncbi.nlm.nih.gov/pubmed/31187731 http://dx.doi.org/10.7554/eLife.47102 |
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author | Box, Kimberly Joyce, Bradley W Devenport, Danelle |
author_facet | Box, Kimberly Joyce, Bradley W Devenport, Danelle |
author_sort | Box, Kimberly |
collection | PubMed |
description | The control of cell fate through oriented cell division is imperative for proper organ development. Basal epidermal progenitor cells divide parallel or perpendicular to the basement membrane to self-renew or produce differentiated stratified layers, but the mechanisms regulating the choice between division orientations are unknown. Using time-lapse imaging to follow divisions and fates of basal progenitors, we find that mouse embryos defective for the planar cell polarity (PCP) gene, Vangl2, exhibit increased perpendicular divisions and hyperthickened epidermis. Surprisingly, this is not due to defective Vangl2 function in the epidermis, but to changes in cell geometry and packing that arise from the open neural tube characteristic of PCP mutants. Through regional variations in epidermal deformation and physical manipulations, we show that local tissue architecture, rather than cortical PCP cues, regulates the decision between symmetric and stratifying divisions, allowing flexibility for basal cells to adapt to the needs of the developing tissue. |
format | Online Article Text |
id | pubmed-6592681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65926812019-06-26 Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis Box, Kimberly Joyce, Bradley W Devenport, Danelle eLife Developmental Biology The control of cell fate through oriented cell division is imperative for proper organ development. Basal epidermal progenitor cells divide parallel or perpendicular to the basement membrane to self-renew or produce differentiated stratified layers, but the mechanisms regulating the choice between division orientations are unknown. Using time-lapse imaging to follow divisions and fates of basal progenitors, we find that mouse embryos defective for the planar cell polarity (PCP) gene, Vangl2, exhibit increased perpendicular divisions and hyperthickened epidermis. Surprisingly, this is not due to defective Vangl2 function in the epidermis, but to changes in cell geometry and packing that arise from the open neural tube characteristic of PCP mutants. Through regional variations in epidermal deformation and physical manipulations, we show that local tissue architecture, rather than cortical PCP cues, regulates the decision between symmetric and stratifying divisions, allowing flexibility for basal cells to adapt to the needs of the developing tissue. eLife Sciences Publications, Ltd 2019-06-12 /pmc/articles/PMC6592681/ /pubmed/31187731 http://dx.doi.org/10.7554/eLife.47102 Text en © 2019, Box et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Box, Kimberly Joyce, Bradley W Devenport, Danelle Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title_full | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title_fullStr | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title_full_unstemmed | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title_short | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
title_sort | epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592681/ https://www.ncbi.nlm.nih.gov/pubmed/31187731 http://dx.doi.org/10.7554/eLife.47102 |
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