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Thermal constraints on in vivo optogenetic manipulations
A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly-used illumination protocols increased temperature by 0.2–2°C and suppressed spiking in multiple br...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592769/ https://www.ncbi.nlm.nih.gov/pubmed/31209378 http://dx.doi.org/10.1038/s41593-019-0422-3 |
| _version_ | 1783429929422028800 |
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| author | Owen, Scott F. Liu, Max H. Kreitzer, Anatol C. |
| author_facet | Owen, Scott F. Liu, Max H. Kreitzer, Anatol C. |
| author_sort | Owen, Scott F. |
| collection | PubMed |
| description | A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly-used illumination protocols increased temperature by 0.2–2°C and suppressed spiking in multiple brain regions. In striatum, light delivery activated an inwardly-rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light delivery parameters is required, as even modest intracranial heating can confound interpretation of optogenetic experiments. |
| format | Online Article Text |
| id | pubmed-6592769 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-65927692019-12-17 Thermal constraints on in vivo optogenetic manipulations Owen, Scott F. Liu, Max H. Kreitzer, Anatol C. Nat Neurosci Article A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly-used illumination protocols increased temperature by 0.2–2°C and suppressed spiking in multiple brain regions. In striatum, light delivery activated an inwardly-rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light delivery parameters is required, as even modest intracranial heating can confound interpretation of optogenetic experiments. 2019-06-17 2019-07 /pmc/articles/PMC6592769/ /pubmed/31209378 http://dx.doi.org/10.1038/s41593-019-0422-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Owen, Scott F. Liu, Max H. Kreitzer, Anatol C. Thermal constraints on in vivo optogenetic manipulations |
| title | Thermal constraints on in vivo optogenetic manipulations |
| title_full | Thermal constraints on in vivo optogenetic manipulations |
| title_fullStr | Thermal constraints on in vivo optogenetic manipulations |
| title_full_unstemmed | Thermal constraints on in vivo optogenetic manipulations |
| title_short | Thermal constraints on in vivo optogenetic manipulations |
| title_sort | thermal constraints on in vivo optogenetic manipulations |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592769/ https://www.ncbi.nlm.nih.gov/pubmed/31209378 http://dx.doi.org/10.1038/s41593-019-0422-3 |
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