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Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans

It has been repeatedly reported that transposable elements (TE) become active and/or mobile in the genomes of replicatively and stress-induced senescent mammalian cells. However, the biological role of senescence-associated transposon activation and its occurrence and relevance in other eukaryotic c...

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Autores principales: Potocki, Leszek, Kuna, Ewelina, Filip, Kamila, Kasprzyk, Beata, Lewinska, Anna, Wnuk, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593122/
https://www.ncbi.nlm.nih.gov/pubmed/30989423
http://dx.doi.org/10.1007/s10522-019-09809-2
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author Potocki, Leszek
Kuna, Ewelina
Filip, Kamila
Kasprzyk, Beata
Lewinska, Anna
Wnuk, Maciej
author_facet Potocki, Leszek
Kuna, Ewelina
Filip, Kamila
Kasprzyk, Beata
Lewinska, Anna
Wnuk, Maciej
author_sort Potocki, Leszek
collection PubMed
description It has been repeatedly reported that transposable elements (TE) become active and/or mobile in the genomes of replicatively and stress-induced senescent mammalian cells. However, the biological role of senescence-associated transposon activation and its occurrence and relevance in other eukaryotic cells remain to be elucidated. In the present study, Candida albicans, a prevalent opportunistic fungal pathogen in humans, was used to analyze changes in gene copy number of selected TE, namely Cirt2, Moa and Cmut1 during long-term culture (up to 90 days). The effects of stress stimuli (fluconazole, hydrogen peroxide, hypochlorite) and ploidy state (haploid, diploid, tetraploid cells) were also considered. An increase in copy number of Cirt2 and Moa was the most accented in tetraploid cells after 90 days of culture that was accompanied by changes in karyotype patterns and slightly more limited growth rate compared to haploid and diploid cells. Stress stimuli did not potentiate TE activity. Elevation in chromosomal DNA breaks was also observed during long-term culture of cells of different ploidy, however this was not correlated with increased TE activity. Our results suggest that increased TE activity may promote genomic diversity and plasticity, and cellular heterogeneity during long-term culture of C. albicans cells.
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spelling pubmed-65931222019-07-11 Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans Potocki, Leszek Kuna, Ewelina Filip, Kamila Kasprzyk, Beata Lewinska, Anna Wnuk, Maciej Biogerontology Research Article It has been repeatedly reported that transposable elements (TE) become active and/or mobile in the genomes of replicatively and stress-induced senescent mammalian cells. However, the biological role of senescence-associated transposon activation and its occurrence and relevance in other eukaryotic cells remain to be elucidated. In the present study, Candida albicans, a prevalent opportunistic fungal pathogen in humans, was used to analyze changes in gene copy number of selected TE, namely Cirt2, Moa and Cmut1 during long-term culture (up to 90 days). The effects of stress stimuli (fluconazole, hydrogen peroxide, hypochlorite) and ploidy state (haploid, diploid, tetraploid cells) were also considered. An increase in copy number of Cirt2 and Moa was the most accented in tetraploid cells after 90 days of culture that was accompanied by changes in karyotype patterns and slightly more limited growth rate compared to haploid and diploid cells. Stress stimuli did not potentiate TE activity. Elevation in chromosomal DNA breaks was also observed during long-term culture of cells of different ploidy, however this was not correlated with increased TE activity. Our results suggest that increased TE activity may promote genomic diversity and plasticity, and cellular heterogeneity during long-term culture of C. albicans cells. Springer Netherlands 2019-04-15 2019 /pmc/articles/PMC6593122/ /pubmed/30989423 http://dx.doi.org/10.1007/s10522-019-09809-2 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Article
Potocki, Leszek
Kuna, Ewelina
Filip, Kamila
Kasprzyk, Beata
Lewinska, Anna
Wnuk, Maciej
Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title_full Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title_fullStr Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title_full_unstemmed Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title_short Activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen Candida albicans
title_sort activation of transposable elements and genetic instability during long-term culture of the human fungal pathogen candida albicans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593122/
https://www.ncbi.nlm.nih.gov/pubmed/30989423
http://dx.doi.org/10.1007/s10522-019-09809-2
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