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Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab
OBJECTIVE: To explore the inhibitory effect of bevacizumab, a vascular endothelial growth factor antibody, on angiogenesis in human osteosarcoma of nude mice. METHODS: Twenty‐one nude mice were inoculated with red fluorescent protein (RFP)‐labeled human osteosarcoma cell line 143B‐RFP, that is, clon...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593177/ https://www.ncbi.nlm.nih.gov/pubmed/27384733 http://dx.doi.org/10.1111/os.12236 |
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author | Zhao, Ze‐xue Li, Xiang Liu, Wei‐dong Liu, Xiao‐zhou Wu, Su‐jia Hu, Xiao‐hui |
author_facet | Zhao, Ze‐xue Li, Xiang Liu, Wei‐dong Liu, Xiao‐zhou Wu, Su‐jia Hu, Xiao‐hui |
author_sort | Zhao, Ze‐xue |
collection | PubMed |
description | OBJECTIVE: To explore the inhibitory effect of bevacizumab, a vascular endothelial growth factor antibody, on angiogenesis in human osteosarcoma of nude mice. METHODS: Twenty‐one nude mice were inoculated with red fluorescent protein (RFP)‐labeled human osteosarcoma cell line 143B‐RFP, that is, clones that expressed RFP in the cytoplasm, and randomly assigned to one of three groups: G1 (Control group, injected with saline solution); G2 (intraperitoneal bevacizumab 2 mg/kg twice per week) and G3 (intraperitoneal bevacizumab 5 mg/kg, twice per week). The tumor‐bearing mice were examined in a fluorescence light box that was illuminated periodically. The primary tumors were measured by fluorescence imaging weekly and their volumes calculated. RESULTS: The mean tumor volumes were significantly smaller in the G3 (186.4 ± 100.8 mm(3)) than the control group (587.0 ± 406.8 mm(3)) (P < 0.05) on Day 31, and again significantly smaller in the G3 (677.3 ± 461.9 mm(3)) than the control group (3162.6 ± 1529.2 mm(3)) on Day 38 (P < 0.01). The average tumor volume in the G2 group was 493.5 ± 425.4 mm(3) on Day 31 and 1870.1 ± 1524.8 mm(3) on Day 38. The effect on tumor volume was greater in the G3 than the G2 group. Three mice in the G2 group, four in the G3 group and four in the control group developed lung metastases that were confirmed by pathological examination; these differences were not statistically significant (P < 0.05). CONCLUSIONS: Bevacizumab exhibits strong antiangiogenesis activity in experimental osteosarcoma in a nude mouse model but does not influence the incidence of lung metastasis. Our findings may have considerable potential for the treatment of osteosarcoma. |
format | Online Article Text |
id | pubmed-6593177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65931772019-09-10 Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab Zhao, Ze‐xue Li, Xiang Liu, Wei‐dong Liu, Xiao‐zhou Wu, Su‐jia Hu, Xiao‐hui Orthop Surg Scientific Article OBJECTIVE: To explore the inhibitory effect of bevacizumab, a vascular endothelial growth factor antibody, on angiogenesis in human osteosarcoma of nude mice. METHODS: Twenty‐one nude mice were inoculated with red fluorescent protein (RFP)‐labeled human osteosarcoma cell line 143B‐RFP, that is, clones that expressed RFP in the cytoplasm, and randomly assigned to one of three groups: G1 (Control group, injected with saline solution); G2 (intraperitoneal bevacizumab 2 mg/kg twice per week) and G3 (intraperitoneal bevacizumab 5 mg/kg, twice per week). The tumor‐bearing mice were examined in a fluorescence light box that was illuminated periodically. The primary tumors were measured by fluorescence imaging weekly and their volumes calculated. RESULTS: The mean tumor volumes were significantly smaller in the G3 (186.4 ± 100.8 mm(3)) than the control group (587.0 ± 406.8 mm(3)) (P < 0.05) on Day 31, and again significantly smaller in the G3 (677.3 ± 461.9 mm(3)) than the control group (3162.6 ± 1529.2 mm(3)) on Day 38 (P < 0.01). The average tumor volume in the G2 group was 493.5 ± 425.4 mm(3) on Day 31 and 1870.1 ± 1524.8 mm(3) on Day 38. The effect on tumor volume was greater in the G3 than the G2 group. Three mice in the G2 group, four in the G3 group and four in the control group developed lung metastases that were confirmed by pathological examination; these differences were not statistically significant (P < 0.05). CONCLUSIONS: Bevacizumab exhibits strong antiangiogenesis activity in experimental osteosarcoma in a nude mouse model but does not influence the incidence of lung metastasis. Our findings may have considerable potential for the treatment of osteosarcoma. John Wiley & Sons Australia, Ltd 2016-07-07 /pmc/articles/PMC6593177/ /pubmed/27384733 http://dx.doi.org/10.1111/os.12236 Text en © 2016 The Authors. Orthopaedic Surgery Published by John Wiley & Sons Australia, Ltd and Chinese Orthopaedic Association This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Scientific Article Zhao, Ze‐xue Li, Xiang Liu, Wei‐dong Liu, Xiao‐zhou Wu, Su‐jia Hu, Xiao‐hui Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title | Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title_full | Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title_fullStr | Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title_full_unstemmed | Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title_short | Inhibition of Growth and Metastasis of Tumor in Nude Mice after Intraperitoneal Injection of Bevacizumab |
title_sort | inhibition of growth and metastasis of tumor in nude mice after intraperitoneal injection of bevacizumab |
topic | Scientific Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593177/ https://www.ncbi.nlm.nih.gov/pubmed/27384733 http://dx.doi.org/10.1111/os.12236 |
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