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The effect of Zika virus infection in the ferret

Although initial observations of infections with the Zika virus describe a mild illness, more recent reports show that infections by Zika result in neurotropism. In 2015, substantial congenital malformations were observed, with numerous infants born with microcephaly in Brazil. To study the underlyi...

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Autores principales: Hutchinson, Elizabeth B., Chatterjee, Mitali, Reyes, Laura, Djankpa, Francis T., Valiant, William G., Dardzinski, Bernard, Mattapallil, Joseph J., Pierpaoli, Carlo, Juliano, Sharon L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593673/
https://www.ncbi.nlm.nih.gov/pubmed/30680733
http://dx.doi.org/10.1002/cne.24640
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author Hutchinson, Elizabeth B.
Chatterjee, Mitali
Reyes, Laura
Djankpa, Francis T.
Valiant, William G.
Dardzinski, Bernard
Mattapallil, Joseph J.
Pierpaoli, Carlo
Juliano, Sharon L.
author_facet Hutchinson, Elizabeth B.
Chatterjee, Mitali
Reyes, Laura
Djankpa, Francis T.
Valiant, William G.
Dardzinski, Bernard
Mattapallil, Joseph J.
Pierpaoli, Carlo
Juliano, Sharon L.
author_sort Hutchinson, Elizabeth B.
collection PubMed
description Although initial observations of infections with the Zika virus describe a mild illness, more recent reports show that infections by Zika result in neurotropism. In 2015, substantial congenital malformations were observed, with numerous infants born with microcephaly in Brazil. To study the underlying mechanism and effects of the disease, it is critical to find suitable animal models. Rodents lack an immune system parallel to humans and also have lissencephalic brains, which are likely to react differently to infections. As the smallest gyrencephalic mammal, ferrets may provide an important animal model to study the Zika virus, as their brains share many characteristics with humans. To evaluate the prospect of using ferrets to study Zika virus infection, we injected seven pregnant jills with the PR strain subcutaneously on gestational day 21, corresponding to the initiation of corticogenesis. These injections resulted in mixed effects. Two animals died of apparent infection, and all kits were resorbed in another animal that did not die. The other four animals remained pregnant until gestational day 40, when the kits were delivered by caesarian section. We evaluated the animals using CT, MRI, diffusion tensor imaging, and immunohistochemistry. The kits displayed a number of features compatible with an infection that impacted both the brain and skull. The outcomes, however, were variable and differed within and across litters, which ranged from the absence of observable abnormalities to prominent changes, suggesting differential vulnerability of kits to infection by the Zika virus or to subsequent mechanisms of neurodevelopmental disruption.
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spelling pubmed-65936732019-07-10 The effect of Zika virus infection in the ferret Hutchinson, Elizabeth B. Chatterjee, Mitali Reyes, Laura Djankpa, Francis T. Valiant, William G. Dardzinski, Bernard Mattapallil, Joseph J. Pierpaoli, Carlo Juliano, Sharon L. J Comp Neurol Research Articles Although initial observations of infections with the Zika virus describe a mild illness, more recent reports show that infections by Zika result in neurotropism. In 2015, substantial congenital malformations were observed, with numerous infants born with microcephaly in Brazil. To study the underlying mechanism and effects of the disease, it is critical to find suitable animal models. Rodents lack an immune system parallel to humans and also have lissencephalic brains, which are likely to react differently to infections. As the smallest gyrencephalic mammal, ferrets may provide an important animal model to study the Zika virus, as their brains share many characteristics with humans. To evaluate the prospect of using ferrets to study Zika virus infection, we injected seven pregnant jills with the PR strain subcutaneously on gestational day 21, corresponding to the initiation of corticogenesis. These injections resulted in mixed effects. Two animals died of apparent infection, and all kits were resorbed in another animal that did not die. The other four animals remained pregnant until gestational day 40, when the kits were delivered by caesarian section. We evaluated the animals using CT, MRI, diffusion tensor imaging, and immunohistochemistry. The kits displayed a number of features compatible with an infection that impacted both the brain and skull. The outcomes, however, were variable and differed within and across litters, which ranged from the absence of observable abnormalities to prominent changes, suggesting differential vulnerability of kits to infection by the Zika virus or to subsequent mechanisms of neurodevelopmental disruption. John Wiley & Sons, Inc. 2019-02-15 2019-07-01 /pmc/articles/PMC6593673/ /pubmed/30680733 http://dx.doi.org/10.1002/cne.24640 Text en © 2019 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Hutchinson, Elizabeth B.
Chatterjee, Mitali
Reyes, Laura
Djankpa, Francis T.
Valiant, William G.
Dardzinski, Bernard
Mattapallil, Joseph J.
Pierpaoli, Carlo
Juliano, Sharon L.
The effect of Zika virus infection in the ferret
title The effect of Zika virus infection in the ferret
title_full The effect of Zika virus infection in the ferret
title_fullStr The effect of Zika virus infection in the ferret
title_full_unstemmed The effect of Zika virus infection in the ferret
title_short The effect of Zika virus infection in the ferret
title_sort effect of zika virus infection in the ferret
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593673/
https://www.ncbi.nlm.nih.gov/pubmed/30680733
http://dx.doi.org/10.1002/cne.24640
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