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Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

BACKGROUND: Neuropeptides are regulators of critical life processes in insects and, due to their high specificity, represent potential targets in the development of greener insecticidal agents. Fundamental to this drive is understanding neuroendocrine pathways that control key physiological processe...

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Autores principales: Alford, Lucy, Marley, Richard, Dornan, Anthony, Pierre, Jean‐Sébastien, Dow, Julian AT, Nachman, Ronald J, Davies, Shireen A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593983/
https://www.ncbi.nlm.nih.gov/pubmed/30734498
http://dx.doi.org/10.1002/ps.5372
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author Alford, Lucy
Marley, Richard
Dornan, Anthony
Pierre, Jean‐Sébastien
Dow, Julian AT
Nachman, Ronald J
Davies, Shireen A
author_facet Alford, Lucy
Marley, Richard
Dornan, Anthony
Pierre, Jean‐Sébastien
Dow, Julian AT
Nachman, Ronald J
Davies, Shireen A
author_sort Alford, Lucy
collection PubMed
description BACKGROUND: Neuropeptides are regulators of critical life processes in insects and, due to their high specificity, represent potential targets in the development of greener insecticidal agents. Fundamental to this drive is understanding neuroendocrine pathways that control key physiological processes in pest insects and the screening of potential analogues. The current study investigated neuropeptide binding sites of kinin and CAPA (CAPA‐1) in the aphids Myzus persicae and Macrosiphum rosae and the effect of biostable analogues on aphid fitness under conditions of desiccation, starvation and thermal (cold) stress. RESULTS: M. persicae and M. rosae displayed identical patterns of neuropeptide receptor mapping along the gut, with the gut musculature representing the main target for kinin and CAPA‐1 action. While kinin receptor binding was observed in the brain and VNC of M. persicae, this was not observed in M. rosae. Furthermore, no CAPA‐1 receptor binding was observed in the brain and VNC of either species. CAP2b/PK analogues (with CAPA receptor cross‐activity) were most effective in reducing aphid fitness under conditions of desiccation and starvation stress, particularly analogues 1895 (2Abf‐Suc‐FGPRLa) and 2129 (2Abf‐Suc‐ATPRIa), which expedited aphid mortality. All analogues, with the exception of 2139‐Ac, were efficient at reducing aphid survival under cold stress, although were equivalent in the strength of their effect. CONCLUSION: In demonstrating the effects of analogues belonging to the CAP2b neuropeptide family and key analogue structures that reduce aphid fitness under stress conditions, this research will feed into the development of second generation analogues and ultimately the development of neuropeptidomimetic‐based insecticidal agents. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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spelling pubmed-65939832019-07-10 Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance Alford, Lucy Marley, Richard Dornan, Anthony Pierre, Jean‐Sébastien Dow, Julian AT Nachman, Ronald J Davies, Shireen A Pest Manag Sci Research Articles BACKGROUND: Neuropeptides are regulators of critical life processes in insects and, due to their high specificity, represent potential targets in the development of greener insecticidal agents. Fundamental to this drive is understanding neuroendocrine pathways that control key physiological processes in pest insects and the screening of potential analogues. The current study investigated neuropeptide binding sites of kinin and CAPA (CAPA‐1) in the aphids Myzus persicae and Macrosiphum rosae and the effect of biostable analogues on aphid fitness under conditions of desiccation, starvation and thermal (cold) stress. RESULTS: M. persicae and M. rosae displayed identical patterns of neuropeptide receptor mapping along the gut, with the gut musculature representing the main target for kinin and CAPA‐1 action. While kinin receptor binding was observed in the brain and VNC of M. persicae, this was not observed in M. rosae. Furthermore, no CAPA‐1 receptor binding was observed in the brain and VNC of either species. CAP2b/PK analogues (with CAPA receptor cross‐activity) were most effective in reducing aphid fitness under conditions of desiccation and starvation stress, particularly analogues 1895 (2Abf‐Suc‐FGPRLa) and 2129 (2Abf‐Suc‐ATPRIa), which expedited aphid mortality. All analogues, with the exception of 2139‐Ac, were efficient at reducing aphid survival under cold stress, although were equivalent in the strength of their effect. CONCLUSION: In demonstrating the effects of analogues belonging to the CAP2b neuropeptide family and key analogue structures that reduce aphid fitness under stress conditions, this research will feed into the development of second generation analogues and ultimately the development of neuropeptidomimetic‐based insecticidal agents. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. John Wiley & Sons, Ltd 2019-03-21 2019-06 /pmc/articles/PMC6593983/ /pubmed/30734498 http://dx.doi.org/10.1002/ps.5372 Text en © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/3.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Alford, Lucy
Marley, Richard
Dornan, Anthony
Pierre, Jean‐Sébastien
Dow, Julian AT
Nachman, Ronald J
Davies, Shireen A
Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title_full Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title_fullStr Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title_full_unstemmed Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title_short Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance
title_sort assessment of neuropeptide binding sites and the impact of biostable kinin and cap2b analogue treatment on aphid (myzus persicae and macrosiphum rosae) stress tolerance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593983/
https://www.ncbi.nlm.nih.gov/pubmed/30734498
http://dx.doi.org/10.1002/ps.5372
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