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Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma

Papillary thyroid carcinoma (PTC) is most common among all thyroid cancers. Multiple genomic alterations occur in PTC, and gene rearrangements are one of them. Here we screened 14 tumors for novel fusion transcripts by RNA‐Seq. Two samples harboring RET/PTC1 and RET/PTC3 rearrangements were positive...

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Autores principales: Pfeifer, Aleksandra, Rusinek, Dagmara, Żebracka‐Gala, Jadwiga, Czarniecka, Agnieszka, Chmielik, Ewa, Zembala‐Nożyńska, Ewa, Wojtaś, Bartosz, Gielniewski, Bartłomiej, Szpak‐Ulczok, Sylwia, Oczko‐Wojciechowska, Małgorzata, Krajewska, Jolanta, Polańska, Joanna, Jarząb, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594006/
https://www.ncbi.nlm.nih.gov/pubmed/30664823
http://dx.doi.org/10.1002/gcc.22737
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author Pfeifer, Aleksandra
Rusinek, Dagmara
Żebracka‐Gala, Jadwiga
Czarniecka, Agnieszka
Chmielik, Ewa
Zembala‐Nożyńska, Ewa
Wojtaś, Bartosz
Gielniewski, Bartłomiej
Szpak‐Ulczok, Sylwia
Oczko‐Wojciechowska, Małgorzata
Krajewska, Jolanta
Polańska, Joanna
Jarząb, Barbara
author_facet Pfeifer, Aleksandra
Rusinek, Dagmara
Żebracka‐Gala, Jadwiga
Czarniecka, Agnieszka
Chmielik, Ewa
Zembala‐Nożyńska, Ewa
Wojtaś, Bartosz
Gielniewski, Bartłomiej
Szpak‐Ulczok, Sylwia
Oczko‐Wojciechowska, Małgorzata
Krajewska, Jolanta
Polańska, Joanna
Jarząb, Barbara
author_sort Pfeifer, Aleksandra
collection PubMed
description Papillary thyroid carcinoma (PTC) is most common among all thyroid cancers. Multiple genomic alterations occur in PTC, and gene rearrangements are one of them. Here we screened 14 tumors for novel fusion transcripts by RNA‐Seq. Two samples harboring RET/PTC1 and RET/PTC3 rearrangements were positive controls whereas the remaining ones were negative regarding the common PTC alterations. We used Sanger sequencing to validate potential fusions. We detected 2 novel potentially oncogenic transcript fusions: TG‐FGFR1 and TRIM33‐NTRK1. We detected 4 novel fusion transcripts of unknown significance accompanying the TRIM33‐NTRK1 fusion: ZSWIM5‐TP53BP2, TAF4B‐WDR1, ABI2‐MTA3, and ARID1B‐PSMA1. Apart from confirming the presence of RET/PTC1 and RET/PTC3 in positive control samples, we also detected known oncogenic fusion transcripts in remaining samples: TFG‐NTRK1, ETV6‐NTRK3, MKRN1‐BRAF, EML4‐ALK, and novel isoform of CCDC6‐RET.
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spelling pubmed-65940062019-07-10 Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma Pfeifer, Aleksandra Rusinek, Dagmara Żebracka‐Gala, Jadwiga Czarniecka, Agnieszka Chmielik, Ewa Zembala‐Nożyńska, Ewa Wojtaś, Bartosz Gielniewski, Bartłomiej Szpak‐Ulczok, Sylwia Oczko‐Wojciechowska, Małgorzata Krajewska, Jolanta Polańska, Joanna Jarząb, Barbara Genes Chromosomes Cancer Research Articles Papillary thyroid carcinoma (PTC) is most common among all thyroid cancers. Multiple genomic alterations occur in PTC, and gene rearrangements are one of them. Here we screened 14 tumors for novel fusion transcripts by RNA‐Seq. Two samples harboring RET/PTC1 and RET/PTC3 rearrangements were positive controls whereas the remaining ones were negative regarding the common PTC alterations. We used Sanger sequencing to validate potential fusions. We detected 2 novel potentially oncogenic transcript fusions: TG‐FGFR1 and TRIM33‐NTRK1. We detected 4 novel fusion transcripts of unknown significance accompanying the TRIM33‐NTRK1 fusion: ZSWIM5‐TP53BP2, TAF4B‐WDR1, ABI2‐MTA3, and ARID1B‐PSMA1. Apart from confirming the presence of RET/PTC1 and RET/PTC3 in positive control samples, we also detected known oncogenic fusion transcripts in remaining samples: TFG‐NTRK1, ETV6‐NTRK3, MKRN1‐BRAF, EML4‐ALK, and novel isoform of CCDC6‐RET. John Wiley & Sons, Inc. 2019-02-18 2019-08 /pmc/articles/PMC6594006/ /pubmed/30664823 http://dx.doi.org/10.1002/gcc.22737 Text en © 2019 The Authors. Genes, Chromosomes & Cancer published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Pfeifer, Aleksandra
Rusinek, Dagmara
Żebracka‐Gala, Jadwiga
Czarniecka, Agnieszka
Chmielik, Ewa
Zembala‐Nożyńska, Ewa
Wojtaś, Bartosz
Gielniewski, Bartłomiej
Szpak‐Ulczok, Sylwia
Oczko‐Wojciechowska, Małgorzata
Krajewska, Jolanta
Polańska, Joanna
Jarząb, Barbara
Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title_full Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title_fullStr Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title_full_unstemmed Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title_short Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma
title_sort novel tg‐fgfr1 and trim33‐ntrk1 transcript fusions in papillary thyroid carcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594006/
https://www.ncbi.nlm.nih.gov/pubmed/30664823
http://dx.doi.org/10.1002/gcc.22737
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