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Effect of osteochondral graft orientation in a biotribological test system

Autologous osteochondral transplantation (AOT) utilizing autografts is a widely used technique for the treatment of small‐to‐medium cartilage defects occurring in knee and ankle joints. The application of viable cartilage and bone ensures proper integration, early weight bearing, as well as restorat...

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Autores principales: Bauer, Christoph, Göçerler, Hakan, Niculescu‐Morzsa, Eugenia, Jeyakumar, Vivek, Stotter, Christoph, Tóth, Ivana, Klestil, Thomas, Franek, Friedrich, Nehrer, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594111/
https://www.ncbi.nlm.nih.gov/pubmed/30690777
http://dx.doi.org/10.1002/jor.24236
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author Bauer, Christoph
Göçerler, Hakan
Niculescu‐Morzsa, Eugenia
Jeyakumar, Vivek
Stotter, Christoph
Tóth, Ivana
Klestil, Thomas
Franek, Friedrich
Nehrer, Stefan
author_facet Bauer, Christoph
Göçerler, Hakan
Niculescu‐Morzsa, Eugenia
Jeyakumar, Vivek
Stotter, Christoph
Tóth, Ivana
Klestil, Thomas
Franek, Friedrich
Nehrer, Stefan
author_sort Bauer, Christoph
collection PubMed
description Autologous osteochondral transplantation (AOT) utilizing autografts is a widely used technique for the treatment of small‐to‐medium cartilage defects occurring in knee and ankle joints. The application of viable cartilage and bone ensures proper integration, early weight bearing, as well as restoration of biomechanical and biotribological properties. However, alignment of the autografts onto the defect site remains a pivotal aspect of reinstating the properties of the joint toward successful autograft integration. This is the first study to perform tests with different orientations of osteochondral grafts in a cartilage‐on‐cartilage test system. The objective was to estimate if there are differences between aligned and 90°‐rotated grafts concerning molecular biological and biomechanical parameters. Tissue viability, assessed by XTT assay indicated lower metabolic activity in tested osteochondral grafts (aligned, p = 0.0148 and 90°‐rotated, p = 0.0760) in favor of a higher anabolic gene expression (aligned, p = 0.0030 and 90°‐rotated, 0.0027). Tissue structure was evaluated by Safranin O histology and microscopic images of the surface. Aligned and 90°‐rotated grafts revealed no apparent differences between proteoglycan content or cracks and fissures on the cartilage surface. Test medium analyzed after tribological tests for their sulfated glycosaminoglycan content revealed no differences (p = 0.3282). During the tests, both the friction coefficient and the relative displacement between the two cartilage surfaces were measured, with no significant difference in both parameters (COF, p = 0.2232 and relative displacement, p = 0.3185). From the methods we deployed, this study can infer that there are no differences between aligned and 90°‐rotated osteochondral grafts after tribological tests in the used ex vivo tissue model. © 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res
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spelling pubmed-65941112019-07-10 Effect of osteochondral graft orientation in a biotribological test system Bauer, Christoph Göçerler, Hakan Niculescu‐Morzsa, Eugenia Jeyakumar, Vivek Stotter, Christoph Tóth, Ivana Klestil, Thomas Franek, Friedrich Nehrer, Stefan J Orthop Res Research Articles Autologous osteochondral transplantation (AOT) utilizing autografts is a widely used technique for the treatment of small‐to‐medium cartilage defects occurring in knee and ankle joints. The application of viable cartilage and bone ensures proper integration, early weight bearing, as well as restoration of biomechanical and biotribological properties. However, alignment of the autografts onto the defect site remains a pivotal aspect of reinstating the properties of the joint toward successful autograft integration. This is the first study to perform tests with different orientations of osteochondral grafts in a cartilage‐on‐cartilage test system. The objective was to estimate if there are differences between aligned and 90°‐rotated grafts concerning molecular biological and biomechanical parameters. Tissue viability, assessed by XTT assay indicated lower metabolic activity in tested osteochondral grafts (aligned, p = 0.0148 and 90°‐rotated, p = 0.0760) in favor of a higher anabolic gene expression (aligned, p = 0.0030 and 90°‐rotated, 0.0027). Tissue structure was evaluated by Safranin O histology and microscopic images of the surface. Aligned and 90°‐rotated grafts revealed no apparent differences between proteoglycan content or cracks and fissures on the cartilage surface. Test medium analyzed after tribological tests for their sulfated glycosaminoglycan content revealed no differences (p = 0.3282). During the tests, both the friction coefficient and the relative displacement between the two cartilage surfaces were measured, with no significant difference in both parameters (COF, p = 0.2232 and relative displacement, p = 0.3185). From the methods we deployed, this study can infer that there are no differences between aligned and 90°‐rotated osteochondral grafts after tribological tests in the used ex vivo tissue model. © 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res John Wiley and Sons Inc. 2019-02-21 2019-03 /pmc/articles/PMC6594111/ /pubmed/30690777 http://dx.doi.org/10.1002/jor.24236 Text en © 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Bauer, Christoph
Göçerler, Hakan
Niculescu‐Morzsa, Eugenia
Jeyakumar, Vivek
Stotter, Christoph
Tóth, Ivana
Klestil, Thomas
Franek, Friedrich
Nehrer, Stefan
Effect of osteochondral graft orientation in a biotribological test system
title Effect of osteochondral graft orientation in a biotribological test system
title_full Effect of osteochondral graft orientation in a biotribological test system
title_fullStr Effect of osteochondral graft orientation in a biotribological test system
title_full_unstemmed Effect of osteochondral graft orientation in a biotribological test system
title_short Effect of osteochondral graft orientation in a biotribological test system
title_sort effect of osteochondral graft orientation in a biotribological test system
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594111/
https://www.ncbi.nlm.nih.gov/pubmed/30690777
http://dx.doi.org/10.1002/jor.24236
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