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Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma

Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression. However, molecular mechanisms of different levels of ZBTB7A are still unclear. It is necessary to search molecular markers which are closely connected with ZBTB7A. We selected NP...

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Detalles Bibliográficos
Autores principales: Liu, Fei, Wei, Jiazhang, Hao, Yanrong, Tang, Fengzhu, Jiao, Wei, Qu, Shenhong, He, Ning, Cai, Yonglin, Lan, Jiao, Yang, Yong, Wang, Yongli, Li, Min, Weng, Jingjin, Li, Bing, Lu, Jinlong, Han, Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594332/
https://www.ncbi.nlm.nih.gov/pubmed/31309112
http://dx.doi.org/10.1155/2019/7246491
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author Liu, Fei
Wei, Jiazhang
Hao, Yanrong
Tang, Fengzhu
Jiao, Wei
Qu, Shenhong
He, Ning
Cai, Yonglin
Lan, Jiao
Yang, Yong
Wang, Yongli
Li, Min
Weng, Jingjin
Li, Bing
Lu, Jinlong
Han, Xing
author_facet Liu, Fei
Wei, Jiazhang
Hao, Yanrong
Tang, Fengzhu
Jiao, Wei
Qu, Shenhong
He, Ning
Cai, Yonglin
Lan, Jiao
Yang, Yong
Wang, Yongli
Li, Min
Weng, Jingjin
Li, Bing
Lu, Jinlong
Han, Xing
author_sort Liu, Fei
collection PubMed
description Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression. However, molecular mechanisms of different levels of ZBTB7A are still unclear. It is necessary to search molecular markers which are closely connected with ZBTB7A. We selected NPC sublines CNE2 with stably transfecting empty plasmid (negative control, NC) and short hair RNA (shRNA) plasmid targeting ZBTB7A as research objectives. Microarray was used to screen differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) via shRNA-CNE2 versus NC-CNE2. Quantitative PCR (qPCR) was used to validate lncRNAs and mRNAs from the sublines, chronic rhinitis, and NPC tissues. Bioinformatics was used to analyze regulatory pathways which were connected with ZBTB7A. The 1501 lncRNAs (long noncoding RNAs) and 1275 differentially expressed mRNAs were upregulated or downregulated over 2-fold. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the upregulated or downregulated carbohydrate and lipid metabolisms probably involved in carcinogenicity of shRNA-CNE2 (P-value cut-off was 0.05). In order to find the molecular mechanisms of ZBTB7A, we validated 12 differentially expressed lncRNAs and their nearby mRNAs by qPCR. Most of the differentially expressed mRNAs are closely connected with carbohydrate and lipid metabolisms in multiply cancers. Furthermore, part of them were validated in NPC and rhinitis tissues by qPCR. As a result, NR_047538, ENST00000442852, and fatty acid synthase (FASN) were closely associated with NPC. ZBTB7A had a positive association with NR_047538 and negative associations with ENST00000442852 and FASN. The results probably provide novel candidate biomarkers for NPC progression with different levels of ZBTB7A.
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spelling pubmed-65943322019-07-15 Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma Liu, Fei Wei, Jiazhang Hao, Yanrong Tang, Fengzhu Jiao, Wei Qu, Shenhong He, Ning Cai, Yonglin Lan, Jiao Yang, Yong Wang, Yongli Li, Min Weng, Jingjin Li, Bing Lu, Jinlong Han, Xing Biomed Res Int Research Article Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression. However, molecular mechanisms of different levels of ZBTB7A are still unclear. It is necessary to search molecular markers which are closely connected with ZBTB7A. We selected NPC sublines CNE2 with stably transfecting empty plasmid (negative control, NC) and short hair RNA (shRNA) plasmid targeting ZBTB7A as research objectives. Microarray was used to screen differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) via shRNA-CNE2 versus NC-CNE2. Quantitative PCR (qPCR) was used to validate lncRNAs and mRNAs from the sublines, chronic rhinitis, and NPC tissues. Bioinformatics was used to analyze regulatory pathways which were connected with ZBTB7A. The 1501 lncRNAs (long noncoding RNAs) and 1275 differentially expressed mRNAs were upregulated or downregulated over 2-fold. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the upregulated or downregulated carbohydrate and lipid metabolisms probably involved in carcinogenicity of shRNA-CNE2 (P-value cut-off was 0.05). In order to find the molecular mechanisms of ZBTB7A, we validated 12 differentially expressed lncRNAs and their nearby mRNAs by qPCR. Most of the differentially expressed mRNAs are closely connected with carbohydrate and lipid metabolisms in multiply cancers. Furthermore, part of them were validated in NPC and rhinitis tissues by qPCR. As a result, NR_047538, ENST00000442852, and fatty acid synthase (FASN) were closely associated with NPC. ZBTB7A had a positive association with NR_047538 and negative associations with ENST00000442852 and FASN. The results probably provide novel candidate biomarkers for NPC progression with different levels of ZBTB7A. Hindawi 2019-06-11 /pmc/articles/PMC6594332/ /pubmed/31309112 http://dx.doi.org/10.1155/2019/7246491 Text en Copyright © 2019 Fei Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Fei
Wei, Jiazhang
Hao, Yanrong
Tang, Fengzhu
Jiao, Wei
Qu, Shenhong
He, Ning
Cai, Yonglin
Lan, Jiao
Yang, Yong
Wang, Yongli
Li, Min
Weng, Jingjin
Li, Bing
Lu, Jinlong
Han, Xing
Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title_full Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title_fullStr Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title_full_unstemmed Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title_short Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma
title_sort long noncoding rnas and messenger rnas expression profiles potentially regulated by zbtb7a in nasopharyngeal carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594332/
https://www.ncbi.nlm.nih.gov/pubmed/31309112
http://dx.doi.org/10.1155/2019/7246491
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