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The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors

Invasive candidiasis, mainly caused by Candida albicans, is a serious healthcare problem with high mortality rates, particularly in immunocompromised patients. Innate immune cells express pathogen recognition receptors (PRRs) including C-type lectin-like receptors (CLRs) that bind C. albicans to ini...

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Autores principales: Thompson, Aiysha, Davies, Luke C., Liao, Chia-Te, da Fonseca, Diogo M., Griffiths, James S., Andrews, Robert, Jones, Adam V., Clement, Mathew, Brown, Gordon D., Humphreys, Ian R., Taylor, Philip R., Orr, Selinda J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594653/
https://www.ncbi.nlm.nih.gov/pubmed/31242262
http://dx.doi.org/10.1371/journal.ppat.1007850
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author Thompson, Aiysha
Davies, Luke C.
Liao, Chia-Te
da Fonseca, Diogo M.
Griffiths, James S.
Andrews, Robert
Jones, Adam V.
Clement, Mathew
Brown, Gordon D.
Humphreys, Ian R.
Taylor, Philip R.
Orr, Selinda J.
author_facet Thompson, Aiysha
Davies, Luke C.
Liao, Chia-Te
da Fonseca, Diogo M.
Griffiths, James S.
Andrews, Robert
Jones, Adam V.
Clement, Mathew
Brown, Gordon D.
Humphreys, Ian R.
Taylor, Philip R.
Orr, Selinda J.
author_sort Thompson, Aiysha
collection PubMed
description Invasive candidiasis, mainly caused by Candida albicans, is a serious healthcare problem with high mortality rates, particularly in immunocompromised patients. Innate immune cells express pathogen recognition receptors (PRRs) including C-type lectin-like receptors (CLRs) that bind C. albicans to initiate an immune response. Multiple CLRs including Dectin-1, Dectin-2 and Mincle have been proposed individually to contribute to the immune response to C. albicans. However how these receptors collaborate to clear a fungal infection is unknown. Herein, we used novel multi-CLR knockout (KO) mice to decipher the individual, collaborative and collective roles of Dectin-1, Dectin-2 and Mincle during systemic C. albicans infection. These studies revealed an unappreciated and profound role for CLR co-operation in anti-fungal immunity. The protective effect of multiple CLRs was markedly greater than any single receptor, and was mediated through inflammatory monocytes via recognition and phagocytosis of C. albicans, and production of C. albicans-induced cytokines and chemokines. These CLRs were dispensable for mediating similar responses from neutrophils, likely due to lower expression of these CLRs on neutrophils compared to inflammatory monocytes. Concurrent deletion of Dectin-1 and Dectin-2, or all three CLRs, resulted in dramatically increased susceptibility to systemic C. albicans infection compared to mice lacking a single CLR. Multi-CLR KO mice were unable to control fungal growth due to an inadequate early inflammatory monocyte-mediated response. In response to excessive fungal growth, the multi-CLR KO mice mounted a hyper-inflammatory response, likely leading to multiple organ failure. Thus, these data reveal a critical role for CLR co-operation in the effective control of C. albicans and maintenance of organ function during infection.
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spelling pubmed-65946532019-07-05 The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors Thompson, Aiysha Davies, Luke C. Liao, Chia-Te da Fonseca, Diogo M. Griffiths, James S. Andrews, Robert Jones, Adam V. Clement, Mathew Brown, Gordon D. Humphreys, Ian R. Taylor, Philip R. Orr, Selinda J. PLoS Pathog Research Article Invasive candidiasis, mainly caused by Candida albicans, is a serious healthcare problem with high mortality rates, particularly in immunocompromised patients. Innate immune cells express pathogen recognition receptors (PRRs) including C-type lectin-like receptors (CLRs) that bind C. albicans to initiate an immune response. Multiple CLRs including Dectin-1, Dectin-2 and Mincle have been proposed individually to contribute to the immune response to C. albicans. However how these receptors collaborate to clear a fungal infection is unknown. Herein, we used novel multi-CLR knockout (KO) mice to decipher the individual, collaborative and collective roles of Dectin-1, Dectin-2 and Mincle during systemic C. albicans infection. These studies revealed an unappreciated and profound role for CLR co-operation in anti-fungal immunity. The protective effect of multiple CLRs was markedly greater than any single receptor, and was mediated through inflammatory monocytes via recognition and phagocytosis of C. albicans, and production of C. albicans-induced cytokines and chemokines. These CLRs were dispensable for mediating similar responses from neutrophils, likely due to lower expression of these CLRs on neutrophils compared to inflammatory monocytes. Concurrent deletion of Dectin-1 and Dectin-2, or all three CLRs, resulted in dramatically increased susceptibility to systemic C. albicans infection compared to mice lacking a single CLR. Multi-CLR KO mice were unable to control fungal growth due to an inadequate early inflammatory monocyte-mediated response. In response to excessive fungal growth, the multi-CLR KO mice mounted a hyper-inflammatory response, likely leading to multiple organ failure. Thus, these data reveal a critical role for CLR co-operation in the effective control of C. albicans and maintenance of organ function during infection. Public Library of Science 2019-06-26 /pmc/articles/PMC6594653/ /pubmed/31242262 http://dx.doi.org/10.1371/journal.ppat.1007850 Text en © 2019 Thompson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Thompson, Aiysha
Davies, Luke C.
Liao, Chia-Te
da Fonseca, Diogo M.
Griffiths, James S.
Andrews, Robert
Jones, Adam V.
Clement, Mathew
Brown, Gordon D.
Humphreys, Ian R.
Taylor, Philip R.
Orr, Selinda J.
The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title_full The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title_fullStr The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title_full_unstemmed The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title_short The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors
title_sort protective effect of inflammatory monocytes during systemic c. albicans infection is dependent on collaboration between c-type lectin-like receptors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594653/
https://www.ncbi.nlm.nih.gov/pubmed/31242262
http://dx.doi.org/10.1371/journal.ppat.1007850
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