Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis

Long non-coding RNAs (lncRNAs) play vital roles in diabetic nephropathy (DN). This research aimed to study the potential role and underlying molecular mechanisms of long non-coding RNA MEG3 in DN. We found that MEG3 was upregulated in DN in vivo and in vitro and could enhance cell fibrosis and infla...

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Autores principales: Zha, Fangfang, Qu, Xiaolu, Tang, Bo, Li, Ji, Wang, Yakun, Zheng, PengXi, Ji, Tingting, Zhu, Chun, Bai, Shoujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594792/
https://www.ncbi.nlm.nih.gov/pubmed/31195367
http://dx.doi.org/10.18632/aging.102011
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author Zha, Fangfang
Qu, Xiaolu
Tang, Bo
Li, Ji
Wang, Yakun
Zheng, PengXi
Ji, Tingting
Zhu, Chun
Bai, Shoujun
author_facet Zha, Fangfang
Qu, Xiaolu
Tang, Bo
Li, Ji
Wang, Yakun
Zheng, PengXi
Ji, Tingting
Zhu, Chun
Bai, Shoujun
author_sort Zha, Fangfang
collection PubMed
description Long non-coding RNAs (lncRNAs) play vital roles in diabetic nephropathy (DN). This research aimed to study the potential role and underlying molecular mechanisms of long non-coding RNA MEG3 in DN. We found that MEG3 was upregulated in DN in vivo and in vitro and could enhance cell fibrosis and inflammatory response in DN. MEG3 functioned as an endogenous sponge for miR-181a in mesangial cells (MCs) via direct targeting and in an Ago2-dependent manner. MiR-181a inhibition promoted MC fibrosis and inflammatory response. In addition, Egr-1 was confirmed as a target gene of miR-181a. Further investigations verified that MEG3 promotes fibrosis and inflammatory response via the miR-181a/Egr-1/TLR4 axis in vitro and in vivo. These results provide new insights into the regulation between MEG3 and the miR-181a/Egr-1/TLR4 signaling pathway during DN progression.
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spelling pubmed-65947922019-07-01 Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis Zha, Fangfang Qu, Xiaolu Tang, Bo Li, Ji Wang, Yakun Zheng, PengXi Ji, Tingting Zhu, Chun Bai, Shoujun Aging (Albany NY) Research Paper Long non-coding RNAs (lncRNAs) play vital roles in diabetic nephropathy (DN). This research aimed to study the potential role and underlying molecular mechanisms of long non-coding RNA MEG3 in DN. We found that MEG3 was upregulated in DN in vivo and in vitro and could enhance cell fibrosis and inflammatory response in DN. MEG3 functioned as an endogenous sponge for miR-181a in mesangial cells (MCs) via direct targeting and in an Ago2-dependent manner. MiR-181a inhibition promoted MC fibrosis and inflammatory response. In addition, Egr-1 was confirmed as a target gene of miR-181a. Further investigations verified that MEG3 promotes fibrosis and inflammatory response via the miR-181a/Egr-1/TLR4 axis in vitro and in vivo. These results provide new insights into the regulation between MEG3 and the miR-181a/Egr-1/TLR4 signaling pathway during DN progression. Impact Journals 2019-06-13 /pmc/articles/PMC6594792/ /pubmed/31195367 http://dx.doi.org/10.18632/aging.102011 Text en Copyright © 2019 Zha et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zha, Fangfang
Qu, Xiaolu
Tang, Bo
Li, Ji
Wang, Yakun
Zheng, PengXi
Ji, Tingting
Zhu, Chun
Bai, Shoujun
Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title_full Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title_fullStr Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title_full_unstemmed Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title_short Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
title_sort long non-coding rna meg3 promotes fibrosis and inflammatory response in diabetic nephropathy via mir-181a/egr-1/tlr4 axis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594792/
https://www.ncbi.nlm.nih.gov/pubmed/31195367
http://dx.doi.org/10.18632/aging.102011
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