Cargando…

Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma

In recent years, a growing body of evidence has provided support for the important role of microRNAs (miRNAs) in the progression of human cancers. A recent study showed that a novel miRNA miR-3650 expression was significantly decreased in hepatocellular carcinoma (HCC). However, the precise role of...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jian, Huang, Wen-Jin, Xi, Hong-Li, Liu, Ling-Yun, Wang, Shu-Tong, Fan, Wen-Zhe, Peng, Bao-Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594810/
https://www.ncbi.nlm.nih.gov/pubmed/31163018
http://dx.doi.org/10.18632/aging.101981
_version_ 1783430303650414592
author Wu, Jian
Huang, Wen-Jin
Xi, Hong-Li
Liu, Ling-Yun
Wang, Shu-Tong
Fan, Wen-Zhe
Peng, Bao-Gang
author_facet Wu, Jian
Huang, Wen-Jin
Xi, Hong-Li
Liu, Ling-Yun
Wang, Shu-Tong
Fan, Wen-Zhe
Peng, Bao-Gang
author_sort Wu, Jian
collection PubMed
description In recent years, a growing body of evidence has provided support for the important role of microRNAs (miRNAs) in the progression of human cancers. A recent study showed that a novel miRNA miR-3650 expression was significantly decreased in hepatocellular carcinoma (HCC). However, the precise role of miR-3650 in HCC have remained poorly understood. In this study, we found that miR-3650 expression was frequently decreased in HCC tissues. Low expression of miR-3650 is positively associated with tumor metastasis and poor survival of HCC patients. Forced expression of miR-3650 significantly inhibited the migration and epithelial-mesenchymal transition (EMT) of HCC cells. Through bioinformatic analysis and luciferase assays, we confirmed that neurofascin (NFASC) is a directly target mRNA of miR-3650. Rescue experiment demonstrated that NAFSC overexpression could partially counteracted the inhibitory effect of miR-3650 in HCC metastasis and EMT. In conclusion, our findings are the first time to demonstrate that reduced expression of miR-3650 in HCC was correlated with tumor metastasis and poor survival. MiR-3650 repressed HCC migration and EMT by directly targeting NFASC. Our findings suggested that miR-3650 may serve as a potential prognostic marker and promising application in HCC therapy.
format Online
Article
Text
id pubmed-6594810
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-65948102019-07-01 Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma Wu, Jian Huang, Wen-Jin Xi, Hong-Li Liu, Ling-Yun Wang, Shu-Tong Fan, Wen-Zhe Peng, Bao-Gang Aging (Albany NY) Research Paper In recent years, a growing body of evidence has provided support for the important role of microRNAs (miRNAs) in the progression of human cancers. A recent study showed that a novel miRNA miR-3650 expression was significantly decreased in hepatocellular carcinoma (HCC). However, the precise role of miR-3650 in HCC have remained poorly understood. In this study, we found that miR-3650 expression was frequently decreased in HCC tissues. Low expression of miR-3650 is positively associated with tumor metastasis and poor survival of HCC patients. Forced expression of miR-3650 significantly inhibited the migration and epithelial-mesenchymal transition (EMT) of HCC cells. Through bioinformatic analysis and luciferase assays, we confirmed that neurofascin (NFASC) is a directly target mRNA of miR-3650. Rescue experiment demonstrated that NAFSC overexpression could partially counteracted the inhibitory effect of miR-3650 in HCC metastasis and EMT. In conclusion, our findings are the first time to demonstrate that reduced expression of miR-3650 in HCC was correlated with tumor metastasis and poor survival. MiR-3650 repressed HCC migration and EMT by directly targeting NFASC. Our findings suggested that miR-3650 may serve as a potential prognostic marker and promising application in HCC therapy. Impact Journals 2019-06-04 /pmc/articles/PMC6594810/ /pubmed/31163018 http://dx.doi.org/10.18632/aging.101981 Text en Copyright © 2019 Wu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wu, Jian
Huang, Wen-Jin
Xi, Hong-Li
Liu, Ling-Yun
Wang, Shu-Tong
Fan, Wen-Zhe
Peng, Bao-Gang
Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title_full Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title_fullStr Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title_full_unstemmed Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title_short Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma
title_sort tumor-suppressive mir-3650 inhibits tumor metastasis by directly targeting nfasc in hepatocellular carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594810/
https://www.ncbi.nlm.nih.gov/pubmed/31163018
http://dx.doi.org/10.18632/aging.101981
work_keys_str_mv AT wujian tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT huangwenjin tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT xihongli tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT liulingyun tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT wangshutong tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT fanwenzhe tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma
AT pengbaogang tumorsuppressivemir3650inhibitstumormetastasisbydirectlytargetingnfascinhepatocellularcarcinoma