Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone

We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatur...

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Autores principales: Khiatah, Bashar, Qi, Meirigeng, Wu, Youjun, Chen, Kuan-Tsen, Perez, Rachel, Valiente, Luis, Omori, Keiko, Isenberg, Jeffrey S., Kandeel, Fouad, Yee, Jiing-Kuan, Al-Abdullah, Ismail H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594947/
https://www.ncbi.nlm.nih.gov/pubmed/31243300
http://dx.doi.org/10.1038/s41598-019-45678-y
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author Khiatah, Bashar
Qi, Meirigeng
Wu, Youjun
Chen, Kuan-Tsen
Perez, Rachel
Valiente, Luis
Omori, Keiko
Isenberg, Jeffrey S.
Kandeel, Fouad
Yee, Jiing-Kuan
Al-Abdullah, Ismail H.
author_facet Khiatah, Bashar
Qi, Meirigeng
Wu, Youjun
Chen, Kuan-Tsen
Perez, Rachel
Valiente, Luis
Omori, Keiko
Isenberg, Jeffrey S.
Kandeel, Fouad
Yee, Jiing-Kuan
Al-Abdullah, Ismail H.
author_sort Khiatah, Bashar
collection PubMed
description We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatures, time intervals and cell and tissue types found it to be robust for identifying these cells. Through high transition zinc binding, the iDTZ compound concentrated in insulin-producing cells and proved effective at delineating zinc levels in vitro.
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spelling pubmed-65949472019-07-03 Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone Khiatah, Bashar Qi, Meirigeng Wu, Youjun Chen, Kuan-Tsen Perez, Rachel Valiente, Luis Omori, Keiko Isenberg, Jeffrey S. Kandeel, Fouad Yee, Jiing-Kuan Al-Abdullah, Ismail H. Sci Rep Article We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatures, time intervals and cell and tissue types found it to be robust for identifying these cells. Through high transition zinc binding, the iDTZ compound concentrated in insulin-producing cells and proved effective at delineating zinc levels in vitro. Nature Publishing Group UK 2019-06-26 /pmc/articles/PMC6594947/ /pubmed/31243300 http://dx.doi.org/10.1038/s41598-019-45678-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Khiatah, Bashar
Qi, Meirigeng
Wu, Youjun
Chen, Kuan-Tsen
Perez, Rachel
Valiente, Luis
Omori, Keiko
Isenberg, Jeffrey S.
Kandeel, Fouad
Yee, Jiing-Kuan
Al-Abdullah, Ismail H.
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title_full Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title_fullStr Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title_full_unstemmed Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title_short Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
title_sort pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed dithizone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594947/
https://www.ncbi.nlm.nih.gov/pubmed/31243300
http://dx.doi.org/10.1038/s41598-019-45678-y
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