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Trends in mass spectrometry imaging for cardiovascular diseases

Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, M...

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Autores principales: Mezger, Stephanie T. P., Mingels, Alma M. A., Bekers, Otto, Cillero-Pastor, Berta, Heeren, Ron M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594994/
https://www.ncbi.nlm.nih.gov/pubmed/30980090
http://dx.doi.org/10.1007/s00216-019-01780-8
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author Mezger, Stephanie T. P.
Mingels, Alma M. A.
Bekers, Otto
Cillero-Pastor, Berta
Heeren, Ron M. A.
author_facet Mezger, Stephanie T. P.
Mingels, Alma M. A.
Bekers, Otto
Cillero-Pastor, Berta
Heeren, Ron M. A.
author_sort Mezger, Stephanie T. P.
collection PubMed
description Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, MALDI-based approaches have been applied to obtain in-depth knowledge of cardiac (dys)function. Here, we discuss the different aspects of the MSI protocols, from sample handling to instrumentation used in cardiovascular research, and critically evaluate these methods. The trend towards structural lipid analysis, identification, and “top-down” protein MSI shows the potential for implementation in (pre)clinical research and complementing the diagnostic tests. Moreover, new insights into disease progression are expected and thereby contribute to the understanding of underlying mechanisms related to cardiovascular diseases. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01780-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-65949942019-07-11 Trends in mass spectrometry imaging for cardiovascular diseases Mezger, Stephanie T. P. Mingels, Alma M. A. Bekers, Otto Cillero-Pastor, Berta Heeren, Ron M. A. Anal Bioanal Chem Trends Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, MALDI-based approaches have been applied to obtain in-depth knowledge of cardiac (dys)function. Here, we discuss the different aspects of the MSI protocols, from sample handling to instrumentation used in cardiovascular research, and critically evaluate these methods. The trend towards structural lipid analysis, identification, and “top-down” protein MSI shows the potential for implementation in (pre)clinical research and complementing the diagnostic tests. Moreover, new insights into disease progression are expected and thereby contribute to the understanding of underlying mechanisms related to cardiovascular diseases. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01780-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-04-12 2019 /pmc/articles/PMC6594994/ /pubmed/30980090 http://dx.doi.org/10.1007/s00216-019-01780-8 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Trends
Mezger, Stephanie T. P.
Mingels, Alma M. A.
Bekers, Otto
Cillero-Pastor, Berta
Heeren, Ron M. A.
Trends in mass spectrometry imaging for cardiovascular diseases
title Trends in mass spectrometry imaging for cardiovascular diseases
title_full Trends in mass spectrometry imaging for cardiovascular diseases
title_fullStr Trends in mass spectrometry imaging for cardiovascular diseases
title_full_unstemmed Trends in mass spectrometry imaging for cardiovascular diseases
title_short Trends in mass spectrometry imaging for cardiovascular diseases
title_sort trends in mass spectrometry imaging for cardiovascular diseases
topic Trends
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594994/
https://www.ncbi.nlm.nih.gov/pubmed/30980090
http://dx.doi.org/10.1007/s00216-019-01780-8
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