Ap(4)A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells

The significance of intracellular Ap(4)A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2(fl/fl)/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap(4)A (≈30-fold) compared with those derived from Nudt2(+/+) mice. Interestingly, the elevated Ap(4)A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8(+) OT-I T cells. Overall, the study denotes prominent impact of Ap(4)A over the functionality of DCs. The Nudt2(fl/fl)/CD11c-cre mice could serve as a useful tool to study the influence of Ap(4)A in the critical immune mechanisms of DCs.