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Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells

The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (act...

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Autores principales: Paillé, Audrey, Charton, Romain, Muguet, Alexia, Griesenbeck, Joachim, Smerdon, Michael J., Conconi, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595284/
https://www.ncbi.nlm.nih.gov/pubmed/31294051
http://dx.doi.org/10.1016/j.dib.2019.104083
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author Paillé, Audrey
Charton, Romain
Muguet, Alexia
Griesenbeck, Joachim
Smerdon, Michael J.
Conconi, Antonio
author_facet Paillé, Audrey
Charton, Romain
Muguet, Alexia
Griesenbeck, Joachim
Smerdon, Michael J.
Conconi, Antonio
author_sort Paillé, Audrey
collection PubMed
description The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by Charton et al. (Mutat. Res.), the data presented here are on the hydroxyurea concentration-dependent inhibition of yeast culture growth, on cell cycle arrest before completion of genome replication, and on the opening of rRNA gene chromatin. As comparison, data are presented for yeast arrested in the G1-phase of the cell cycle by the pheromone α-factor.
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spelling pubmed-65952842019-07-10 Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells Paillé, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J. Conconi, Antonio Data Brief Biochemistry, Genetics and Molecular Biology The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by Charton et al. (Mutat. Res.), the data presented here are on the hydroxyurea concentration-dependent inhibition of yeast culture growth, on cell cycle arrest before completion of genome replication, and on the opening of rRNA gene chromatin. As comparison, data are presented for yeast arrested in the G1-phase of the cell cycle by the pheromone α-factor. Elsevier 2019-05-31 /pmc/articles/PMC6595284/ /pubmed/31294051 http://dx.doi.org/10.1016/j.dib.2019.104083 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Paillé, Audrey
Charton, Romain
Muguet, Alexia
Griesenbeck, Joachim
Smerdon, Michael J.
Conconi, Antonio
Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title_full Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title_fullStr Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title_full_unstemmed Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title_short Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
title_sort analyses of rrna gene chromatin in cell cycle arrested saccharomyces cerevisiae cells
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595284/
https://www.ncbi.nlm.nih.gov/pubmed/31294051
http://dx.doi.org/10.1016/j.dib.2019.104083
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