Cargando…
Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells
The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (act...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595284/ https://www.ncbi.nlm.nih.gov/pubmed/31294051 http://dx.doi.org/10.1016/j.dib.2019.104083 |
_version_ | 1783430373951143936 |
---|---|
author | Paillé, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J. Conconi, Antonio |
author_facet | Paillé, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J. Conconi, Antonio |
author_sort | Paillé, Audrey |
collection | PubMed |
description | The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by Charton et al. (Mutat. Res.), the data presented here are on the hydroxyurea concentration-dependent inhibition of yeast culture growth, on cell cycle arrest before completion of genome replication, and on the opening of rRNA gene chromatin. As comparison, data are presented for yeast arrested in the G1-phase of the cell cycle by the pheromone α-factor. |
format | Online Article Text |
id | pubmed-6595284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-65952842019-07-10 Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells Paillé, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J. Conconi, Antonio Data Brief Biochemistry, Genetics and Molecular Biology The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150–200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by Charton et al. (Mutat. Res.), the data presented here are on the hydroxyurea concentration-dependent inhibition of yeast culture growth, on cell cycle arrest before completion of genome replication, and on the opening of rRNA gene chromatin. As comparison, data are presented for yeast arrested in the G1-phase of the cell cycle by the pheromone α-factor. Elsevier 2019-05-31 /pmc/articles/PMC6595284/ /pubmed/31294051 http://dx.doi.org/10.1016/j.dib.2019.104083 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Paillé, Audrey Charton, Romain Muguet, Alexia Griesenbeck, Joachim Smerdon, Michael J. Conconi, Antonio Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title | Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title_full | Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title_fullStr | Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title_full_unstemmed | Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title_short | Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells |
title_sort | analyses of rrna gene chromatin in cell cycle arrested saccharomyces cerevisiae cells |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595284/ https://www.ncbi.nlm.nih.gov/pubmed/31294051 http://dx.doi.org/10.1016/j.dib.2019.104083 |
work_keys_str_mv | AT pailleaudrey analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells AT chartonromain analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells AT muguetalexia analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells AT griesenbeckjoachim analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells AT smerdonmichaelj analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells AT conconiantonio analysesofrrnagenechromatinincellcyclearrestedsaccharomycescerevisiaecells |