Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium

INTRODUCTION: It is unknown if ANO‐1 is expressed in the heart, though the presence of a calcium‐activated chloride current has been proposed to mediate some cardiac dysrhythmias. Furthermore, a specific cell type termed telocytes, morphologically mimicking Cajal cells which use ANO‐1 to modulate th...

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Autores principales: DeSimone, Christopher V., McLeod, Christopher J., Gomez Pinilla, Pedro J., Beyder, Arthur, Farrugia, Gianrico, Asirvatham, Samuel J., Kapa, Suraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595329/
https://www.ncbi.nlm.nih.gov/pubmed/31293701
http://dx.doi.org/10.1002/joa3.12176
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author DeSimone, Christopher V.
McLeod, Christopher J.
Gomez Pinilla, Pedro J.
Beyder, Arthur
Farrugia, Gianrico
Asirvatham, Samuel J.
Kapa, Suraj
author_facet DeSimone, Christopher V.
McLeod, Christopher J.
Gomez Pinilla, Pedro J.
Beyder, Arthur
Farrugia, Gianrico
Asirvatham, Samuel J.
Kapa, Suraj
author_sort DeSimone, Christopher V.
collection PubMed
description INTRODUCTION: It is unknown if ANO‐1 is expressed in the heart, though the presence of a calcium‐activated chloride current has been proposed to mediate some cardiac dysrhythmias. Furthermore, a specific cell type termed telocytes, morphologically mimicking Cajal cells which use ANO‐1 to modulate their pacemaker activity in the gut, have been described in the heart. We therefore sought to determine whether this channel is expressed in the canine heart. METHODS: Myocardium was sampled from the ventricles of five canines. Sections were labeled with anti‐Kit and anti‐ANO‐1 antibodies. Slides were reviewed by four investigators looking at cell morphology, distribution, and co‐localization. Identification of telocytes was based on criteria including morphology, Kit positivity (+), and ANO‐1 positivity (+). RESULTS: Clusters of cells meeting criteria for telocytes were seen in the epicardium, sub‐epicardium, and mid‐myocardium. A small subset of cells that were morphologically similar to myocytes was ANO‐1 (+) but Kit (−). In total, three different cell classes were found: (i) Kit (+), ANO‐1 (+) cells with the appearance of telocytes; (ii) Kit (+), ANO‐1 (−) cells; and (iii) Kit (−), ANO‐1 (+) cells with the morphologic appearance of cardiac myocytes. CONCLUSIONS: Telocytes are present in the canine ventricle and express ANO‐1. These data merit further study to elucidate the functional expression of these channels in the heart and whether they may be targets for cardiac arrhythmias.
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spelling pubmed-65953292019-07-10 Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium DeSimone, Christopher V. McLeod, Christopher J. Gomez Pinilla, Pedro J. Beyder, Arthur Farrugia, Gianrico Asirvatham, Samuel J. Kapa, Suraj J Arrhythm Original Articles INTRODUCTION: It is unknown if ANO‐1 is expressed in the heart, though the presence of a calcium‐activated chloride current has been proposed to mediate some cardiac dysrhythmias. Furthermore, a specific cell type termed telocytes, morphologically mimicking Cajal cells which use ANO‐1 to modulate their pacemaker activity in the gut, have been described in the heart. We therefore sought to determine whether this channel is expressed in the canine heart. METHODS: Myocardium was sampled from the ventricles of five canines. Sections were labeled with anti‐Kit and anti‐ANO‐1 antibodies. Slides were reviewed by four investigators looking at cell morphology, distribution, and co‐localization. Identification of telocytes was based on criteria including morphology, Kit positivity (+), and ANO‐1 positivity (+). RESULTS: Clusters of cells meeting criteria for telocytes were seen in the epicardium, sub‐epicardium, and mid‐myocardium. A small subset of cells that were morphologically similar to myocytes was ANO‐1 (+) but Kit (−). In total, three different cell classes were found: (i) Kit (+), ANO‐1 (+) cells with the appearance of telocytes; (ii) Kit (+), ANO‐1 (−) cells; and (iii) Kit (−), ANO‐1 (+) cells with the morphologic appearance of cardiac myocytes. CONCLUSIONS: Telocytes are present in the canine ventricle and express ANO‐1. These data merit further study to elucidate the functional expression of these channels in the heart and whether they may be targets for cardiac arrhythmias. John Wiley and Sons Inc. 2019-04-05 /pmc/articles/PMC6595329/ /pubmed/31293701 http://dx.doi.org/10.1002/joa3.12176 Text en © 2019 The Authors. Journal of Arrhythmia published by John Wiley & Sons Australia, Ltd on behalf of the Japanese Heart Rhythm Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
DeSimone, Christopher V.
McLeod, Christopher J.
Gomez Pinilla, Pedro J.
Beyder, Arthur
Farrugia, Gianrico
Asirvatham, Samuel J.
Kapa, Suraj
Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title_full Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title_fullStr Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title_full_unstemmed Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title_short Telocytes express ANO‐1‐encoded chloride channels in canine ventricular myocardium
title_sort telocytes express ano‐1‐encoded chloride channels in canine ventricular myocardium
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595329/
https://www.ncbi.nlm.nih.gov/pubmed/31293701
http://dx.doi.org/10.1002/joa3.12176
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