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Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)

The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking wa...

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Autores principales: Erdogan, Innocentia G., Mekuto, Lukhanyo, Ntwampe, Seteno K.O., Fosso-Kankeu, Elvis, Waanders, Frans B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595409/
https://www.ncbi.nlm.nih.gov/pubmed/31294068
http://dx.doi.org/10.1016/j.dib.2019.104135
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author Erdogan, Innocentia G.
Mekuto, Lukhanyo
Ntwampe, Seteno K.O.
Fosso-Kankeu, Elvis
Waanders, Frans B.
author_facet Erdogan, Innocentia G.
Mekuto, Lukhanyo
Ntwampe, Seteno K.O.
Fosso-Kankeu, Elvis
Waanders, Frans B.
author_sort Erdogan, Innocentia G.
collection PubMed
description The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina(®) reaction kits as per manufactures guidelines and sequenced using the illumina(®) MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.
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spelling pubmed-65954092019-07-10 Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep) Erdogan, Innocentia G. Mekuto, Lukhanyo Ntwampe, Seteno K.O. Fosso-Kankeu, Elvis Waanders, Frans B. Data Brief Biochemistry, Genetics and Molecular Biology The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1–V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina(®) reaction kits as per manufactures guidelines and sequenced using the illumina(®) MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified. Elsevier 2019-06-11 /pmc/articles/PMC6595409/ /pubmed/31294068 http://dx.doi.org/10.1016/j.dib.2019.104135 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Erdogan, Innocentia G.
Mekuto, Lukhanyo
Ntwampe, Seteno K.O.
Fosso-Kankeu, Elvis
Waanders, Frans B.
Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title_full Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title_fullStr Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title_full_unstemmed Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title_short Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)
title_sort metagenomic profiling dataset of bacterial communities of a drinking water supply system (dwss) in the arid namaqualand region, south africa: source (lower orange river) to point-of-use (o'kiep)
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595409/
https://www.ncbi.nlm.nih.gov/pubmed/31294068
http://dx.doi.org/10.1016/j.dib.2019.104135
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