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Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64

BACKGROUND: Rice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to mana...

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Autores principales: Yang, Meng, Cheng, Ling, Yan, Liuhui, Shu, Wan, Wang, Xinyi, Qiu, Yongfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595561/
https://www.ncbi.nlm.nih.gov/pubmed/31297040
http://dx.doi.org/10.1186/s41065-019-0098-4
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author Yang, Meng
Cheng, Ling
Yan, Liuhui
Shu, Wan
Wang, Xinyi
Qiu, Yongfu
author_facet Yang, Meng
Cheng, Ling
Yan, Liuhui
Shu, Wan
Wang, Xinyi
Qiu, Yongfu
author_sort Yang, Meng
collection PubMed
description BACKGROUND: Rice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice. RESULTS: Resistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated Bph37. Moreover, Bph1 was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying Bph37 exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects. CONCLUSIONS: We mapped one major BPH resistance gene Bph37 in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs.
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spelling pubmed-65955612019-07-11 Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64 Yang, Meng Cheng, Ling Yan, Liuhui Shu, Wan Wang, Xinyi Qiu, Yongfu Hereditas Research BACKGROUND: Rice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice. RESULTS: Resistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated Bph37. Moreover, Bph1 was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying Bph37 exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects. CONCLUSIONS: We mapped one major BPH resistance gene Bph37 in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs. BioMed Central 2019-06-26 /pmc/articles/PMC6595561/ /pubmed/31297040 http://dx.doi.org/10.1186/s41065-019-0098-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yang, Meng
Cheng, Ling
Yan, Liuhui
Shu, Wan
Wang, Xinyi
Qiu, Yongfu
Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title_full Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title_fullStr Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title_full_unstemmed Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title_short Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64
title_sort mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety ir64
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595561/
https://www.ncbi.nlm.nih.gov/pubmed/31297040
http://dx.doi.org/10.1186/s41065-019-0098-4
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