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Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits
BACKGROUND: Endothelial dysfunction, the initial pathogenic factor in atherosclerosis, can be alleviated via transient limb ischemia. We observed the effects of regular transient limb ischemia (RTLI) on atherosclerosis in hypercholesterolemic rabbits. METHODS: Twenty-eight rabbits were randomized to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595873/ https://www.ncbi.nlm.nih.gov/pubmed/30870265 http://dx.doi.org/10.1097/CM9.0000000000000204 |
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author | Zhou, Yan Zhang, Nan-Rong Zheng, Zhi-Nan Yang, Yi Lyu, Bao-Feng Wang, Hong-Li Jin, San-Qing |
author_facet | Zhou, Yan Zhang, Nan-Rong Zheng, Zhi-Nan Yang, Yi Lyu, Bao-Feng Wang, Hong-Li Jin, San-Qing |
author_sort | Zhou, Yan |
collection | PubMed |
description | BACKGROUND: Endothelial dysfunction, the initial pathogenic factor in atherosclerosis, can be alleviated via transient limb ischemia. We observed the effects of regular transient limb ischemia (RTLI) on atherosclerosis in hypercholesterolemic rabbits. METHODS: Twenty-eight rabbits were randomized to control, cholesterol, sham, ischemia groups (n = 7 each) between October 2010 and March 2011. They were fed a normal diet in the control group and hypercholesterolemic diet in other groups for 12 weeks. Six cycles of RTLI were performed once per day on the ischemia group. Serum samples were prepared to measure the total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) before the experiment (W0), at the end of weeks 4, 8, 12 (W4, W8, W12). The whole aorta was harvested at W12 and stained using Sudan IV to identify the plaque. The plaque area was measured using Image J. Results were analyzed by analysis of variance or rank sum test. RESULTS: Concentrations of TC in the cholesterol group were higher than those in the control group at W4 (29.60 [23.75, 39.30] vs. 1.00 [0.80, 1.55], Z = –2.745, P = 0.006), W8 (41.78 [28.08, 47.37] vs. 0.35 [0.10, 0.68], Z = –2.739, P = 0.006), W12 (48.32 [40.04, 48.95] vs. 0.61 [0.50, 0.86], Z = –2.739, P = 0.006). Similar results were obtained for HDL-C and LDL-C. Serum concentrations of TC, HDL-C, and LDL-C in the hypercholesterolemic groups had no differences (all P > 0.05). The percentage of plaque area in the cholesterol group was higher than that in the control group (47.22 ± 23.89% vs. 0, Z = –2.986, P = 0.003). Square root of the percentage of plaque area was smaller in the ischemia group than that in the cholesterol (0.44 ± 0.13 vs. 0.67 ± 0.18, P = 0.014) or sham groups (0.44 ± 0.13 vs. 0.61 ± 0.12, P = 0.049). CONCLUSION: In hypercholesterolemic rabbits, RTLI might prevent atherosclerosis progression by reducing the percentage of plaque area. |
format | Online Article Text |
id | pubmed-6595873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-65958732019-07-02 Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits Zhou, Yan Zhang, Nan-Rong Zheng, Zhi-Nan Yang, Yi Lyu, Bao-Feng Wang, Hong-Li Jin, San-Qing Chin Med J (Engl) Original Articles BACKGROUND: Endothelial dysfunction, the initial pathogenic factor in atherosclerosis, can be alleviated via transient limb ischemia. We observed the effects of regular transient limb ischemia (RTLI) on atherosclerosis in hypercholesterolemic rabbits. METHODS: Twenty-eight rabbits were randomized to control, cholesterol, sham, ischemia groups (n = 7 each) between October 2010 and March 2011. They were fed a normal diet in the control group and hypercholesterolemic diet in other groups for 12 weeks. Six cycles of RTLI were performed once per day on the ischemia group. Serum samples were prepared to measure the total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) before the experiment (W0), at the end of weeks 4, 8, 12 (W4, W8, W12). The whole aorta was harvested at W12 and stained using Sudan IV to identify the plaque. The plaque area was measured using Image J. Results were analyzed by analysis of variance or rank sum test. RESULTS: Concentrations of TC in the cholesterol group were higher than those in the control group at W4 (29.60 [23.75, 39.30] vs. 1.00 [0.80, 1.55], Z = –2.745, P = 0.006), W8 (41.78 [28.08, 47.37] vs. 0.35 [0.10, 0.68], Z = –2.739, P = 0.006), W12 (48.32 [40.04, 48.95] vs. 0.61 [0.50, 0.86], Z = –2.739, P = 0.006). Similar results were obtained for HDL-C and LDL-C. Serum concentrations of TC, HDL-C, and LDL-C in the hypercholesterolemic groups had no differences (all P > 0.05). The percentage of plaque area in the cholesterol group was higher than that in the control group (47.22 ± 23.89% vs. 0, Z = –2.986, P = 0.003). Square root of the percentage of plaque area was smaller in the ischemia group than that in the cholesterol (0.44 ± 0.13 vs. 0.67 ± 0.18, P = 0.014) or sham groups (0.44 ± 0.13 vs. 0.61 ± 0.12, P = 0.049). CONCLUSION: In hypercholesterolemic rabbits, RTLI might prevent atherosclerosis progression by reducing the percentage of plaque area. Wolters Kluwer Health 2019-05-05 2019-05-05 /pmc/articles/PMC6595873/ /pubmed/30870265 http://dx.doi.org/10.1097/CM9.0000000000000204 Text en Copyright © 2019 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | Original Articles Zhou, Yan Zhang, Nan-Rong Zheng, Zhi-Nan Yang, Yi Lyu, Bao-Feng Wang, Hong-Li Jin, San-Qing Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title | Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title_full | Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title_fullStr | Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title_full_unstemmed | Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title_short | Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
title_sort | regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6595873/ https://www.ncbi.nlm.nih.gov/pubmed/30870265 http://dx.doi.org/10.1097/CM9.0000000000000204 |
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