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The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes

The programmed cell death (PDCD) family plays a significant role in the regulation of cell survival and apoptotic cell death. However, the evolution, distribution and role of the PDCD family in lampreys have not been revealed. Thus, we identified the PDCD gene family in the lamprey genome and classi...

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Autores principales: Guan, Xin, Lu, Jiali, Sun, Feng, Li, Qingwei, Pang, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6596451/
https://www.ncbi.nlm.nih.gov/pubmed/31281315
http://dx.doi.org/10.3389/fimmu.2019.01382
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author Guan, Xin
Lu, Jiali
Sun, Feng
Li, Qingwei
Pang, Yue
author_facet Guan, Xin
Lu, Jiali
Sun, Feng
Li, Qingwei
Pang, Yue
author_sort Guan, Xin
collection PubMed
description The programmed cell death (PDCD) family plays a significant role in the regulation of cell survival and apoptotic cell death. However, the evolution, distribution and role of the PDCD family in lampreys have not been revealed. Thus, we identified the PDCD gene family in the lamprey genome and classified the genes into five subfamilies based on orthologs of the genes, conserved synteny, functional domains, phylogenetic tree, and conserved motifs. The distribution of the lamprey PDCD family and the immune response of the PDCD family in lampreys stimulated by different pathogens were also demonstrated. In addition, we investigated the molecular function of lamprey PDCD2, PDCD5, and PDCD10. Our studies showed that the recombinant lamprey PDCD5 protein and transfection of the L-PDCD5 gene induced cell apoptosis, upregulated the expression of the associated X protein (BAX) and TP53 and downregulated the expression of B cell lymphoma 2 (BCL-2) independent of Caspase 3. In contrast, lamprey PDCD10 suppressed apoptosis in response to cis-diaminedichloro-platinum (II) stimuli. Our phylogenetic and functional data not only provide a better understanding of the evolution of lamprey PDCD genes but also reveal the conservation of PDCD genes in apoptosis. Overall, our results provide a novel perspective on lamprey immune regulation mediated by the PDCD family.
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spelling pubmed-65964512019-07-05 The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes Guan, Xin Lu, Jiali Sun, Feng Li, Qingwei Pang, Yue Front Immunol Immunology The programmed cell death (PDCD) family plays a significant role in the regulation of cell survival and apoptotic cell death. However, the evolution, distribution and role of the PDCD family in lampreys have not been revealed. Thus, we identified the PDCD gene family in the lamprey genome and classified the genes into five subfamilies based on orthologs of the genes, conserved synteny, functional domains, phylogenetic tree, and conserved motifs. The distribution of the lamprey PDCD family and the immune response of the PDCD family in lampreys stimulated by different pathogens were also demonstrated. In addition, we investigated the molecular function of lamprey PDCD2, PDCD5, and PDCD10. Our studies showed that the recombinant lamprey PDCD5 protein and transfection of the L-PDCD5 gene induced cell apoptosis, upregulated the expression of the associated X protein (BAX) and TP53 and downregulated the expression of B cell lymphoma 2 (BCL-2) independent of Caspase 3. In contrast, lamprey PDCD10 suppressed apoptosis in response to cis-diaminedichloro-platinum (II) stimuli. Our phylogenetic and functional data not only provide a better understanding of the evolution of lamprey PDCD genes but also reveal the conservation of PDCD genes in apoptosis. Overall, our results provide a novel perspective on lamprey immune regulation mediated by the PDCD family. Frontiers Media S.A. 2019-06-20 /pmc/articles/PMC6596451/ /pubmed/31281315 http://dx.doi.org/10.3389/fimmu.2019.01382 Text en Copyright © 2019 Guan, Lu, Sun, Li and Pang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Guan, Xin
Lu, Jiali
Sun, Feng
Li, Qingwei
Pang, Yue
The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title_full The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title_fullStr The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title_full_unstemmed The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title_short The Molecular Evolution and Functional Divergence of Lamprey Programmed Cell Death Genes
title_sort molecular evolution and functional divergence of lamprey programmed cell death genes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6596451/
https://www.ncbi.nlm.nih.gov/pubmed/31281315
http://dx.doi.org/10.3389/fimmu.2019.01382
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