DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies

Microbial ecology studies are often performed through extraction of metagenomic DNA followed by amplification and sequencing of a marker. It is known that each step may bias the results. These biases have been explored for the study of bacterial communities, but rarely for fungi. Our aim was therefo...

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Detalles Bibliográficos
Autores principales: Frau, Alessandra, Kenny, John G., Lenzi, Luca, Campbell, Barry J., Ijaz, Umer Z., Duckworth, Carrie A., Burkitt, Michael D., Hall, Neil, Anson, Jim, Darby, Alistair C., Probert, Christopher S. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597572/
https://www.ncbi.nlm.nih.gov/pubmed/31249384
http://dx.doi.org/10.1038/s41598-019-44974-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597572/
https://www.ncbi.nlm.nih.gov/pubmed/31249384
http://dx.doi.org/10.1038/s41598-019-44974-x

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