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DNA extraction and amplicon production strategies deeply inf luence the outcome of gut mycobiome studies
Microbial ecology studies are often performed through extraction of metagenomic DNA followed by amplification and sequencing of a marker. It is known that each step may bias the results. These biases have been explored for the study of bacterial communities, but rarely for fungi. Our aim was therefo...
Autores principales: | Frau, Alessandra, Kenny, John G., Lenzi, Luca, Campbell, Barry J., Ijaz, Umer Z., Duckworth, Carrie A., Burkitt, Michael D., Hall, Neil, Anson, Jim, Darby, Alistair C., Probert, Christopher S. J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597572/ https://www.ncbi.nlm.nih.gov/pubmed/31249384 http://dx.doi.org/10.1038/s41598-019-44974-x |
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