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Comparison of engineered Escherichia coli AF1000 and BL21 strains for (R)-3-hydroxybutyrate production in fed-batch cultivation

Accumulation of acetate is a limiting factor in recombinant production of (R)-3-hydroxybutyrate (3HB) by Escherichia coli in high-cell-density processes. To alleviate this limitation, this study investigated two approaches: (i) deletion of phosphotransacetylase (pta), pyruvate oxidase (poxB), and/or...

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Detalles Bibliográficos
Autores principales:	Perez-Zabaleta, Mariel, Guevara-Martínez, Mónica, Gustavsson, Martin, Quillaguamán, Jorge, Larsson, Gen, van Maris, Antonius J. A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2019
Materias:	Biotechnological Products and Process Engineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597613/ https://www.ncbi.nlm.nih.gov/pubmed/31104101 http://dx.doi.org/10.1007/s00253-019-09876-y

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