Cargando…

Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure

A joint metabolomic and lipidomic workflow is used to account for a potential effect of millimeter waves (MMW) around 60 GHz on biological tissues. For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper...

Descripción completa

Detalles Bibliográficos
Autores principales: Le Pogam, Pierre, Le Page, Yann, Habauzit, Denis, Doué, Mickael, Zhadobov, Maxim, Sauleau, Ronan, Le Dréan, Yves, Rondeau, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597695/
https://www.ncbi.nlm.nih.gov/pubmed/31249327
http://dx.doi.org/10.1038/s41598-019-45662-6
_version_ 1783430633384574976
author Le Pogam, Pierre
Le Page, Yann
Habauzit, Denis
Doué, Mickael
Zhadobov, Maxim
Sauleau, Ronan
Le Dréan, Yves
Rondeau, David
author_facet Le Pogam, Pierre
Le Page, Yann
Habauzit, Denis
Doué, Mickael
Zhadobov, Maxim
Sauleau, Ronan
Le Dréan, Yves
Rondeau, David
author_sort Le Pogam, Pierre
collection PubMed
description A joint metabolomic and lipidomic workflow is used to account for a potential effect of millimeter waves (MMW) around 60 GHz on biological tissues. For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper local exposure limit for general public in the context of a wide scale deployment of MMW technologies and devices. After a 24h-exposure, endo- and extracellular extracts were recovered to be submitted to an integrative UPLC-Q-Exactive metabolomic and lipidomic workflow. R-XCMS data processing and subsequent statistical treatment led to emphasize a limited number of altered features in lipidomic sequences and in intracellular metabolomic analyses, whatever the ionization mode (i.e 0 to 6 dysregulated features). Conversely, important dysregulations could be reported in extracellular metabolomic profiles with 111 and 99 frames being altered upon MMW exposure in positive and negative polarities, respectively. This unexpected extent of modifications can hardly stem from the mild changes that could be reported throughout transcriptomics studies, leading us to hypothesize that MMW might alter the permeability of cell membranes, as reported elsewhere.
format Online
Article
Text
id pubmed-6597695
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65976952019-07-09 Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure Le Pogam, Pierre Le Page, Yann Habauzit, Denis Doué, Mickael Zhadobov, Maxim Sauleau, Ronan Le Dréan, Yves Rondeau, David Sci Rep Article A joint metabolomic and lipidomic workflow is used to account for a potential effect of millimeter waves (MMW) around 60 GHz on biological tissues. For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper local exposure limit for general public in the context of a wide scale deployment of MMW technologies and devices. After a 24h-exposure, endo- and extracellular extracts were recovered to be submitted to an integrative UPLC-Q-Exactive metabolomic and lipidomic workflow. R-XCMS data processing and subsequent statistical treatment led to emphasize a limited number of altered features in lipidomic sequences and in intracellular metabolomic analyses, whatever the ionization mode (i.e 0 to 6 dysregulated features). Conversely, important dysregulations could be reported in extracellular metabolomic profiles with 111 and 99 frames being altered upon MMW exposure in positive and negative polarities, respectively. This unexpected extent of modifications can hardly stem from the mild changes that could be reported throughout transcriptomics studies, leading us to hypothesize that MMW might alter the permeability of cell membranes, as reported elsewhere. Nature Publishing Group UK 2019-06-27 /pmc/articles/PMC6597695/ /pubmed/31249327 http://dx.doi.org/10.1038/s41598-019-45662-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Le Pogam, Pierre
Le Page, Yann
Habauzit, Denis
Doué, Mickael
Zhadobov, Maxim
Sauleau, Ronan
Le Dréan, Yves
Rondeau, David
Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title_full Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title_fullStr Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title_full_unstemmed Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title_short Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
title_sort untargeted metabolomics unveil alterations of biomembranes permeability in human hacat keratinocytes upon 60 ghz millimeter-wave exposure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597695/
https://www.ncbi.nlm.nih.gov/pubmed/31249327
http://dx.doi.org/10.1038/s41598-019-45662-6
work_keys_str_mv AT lepogampierre untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT lepageyann untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT habauzitdenis untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT douemickael untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT zhadobovmaxim untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT sauleauronan untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT ledreanyves untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure
AT rondeaudavid untargetedmetabolomicsunveilalterationsofbiomembranespermeabilityinhumanhacatkeratinocytesupon60ghzmillimeterwaveexposure