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Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line
BACKGROUND: The human hematopoietic stem/progenitor cell 117 (HSPC117) protein is involved in many important biological processes. OBJECTIVE: This study was designed to identify the level of HSPC117 mRNA expression in 10 min pig tissue samples and HSPC117 subcellular localization in the PK15 cell li...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598026/ https://www.ncbi.nlm.nih.gov/pubmed/31045548 http://dx.doi.org/10.3233/THC-199028 |
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author | Ma, Hong Fu, Bo Zhang, Xu Wang, Liang Li, Zhongqiu Liu, Di |
author_facet | Ma, Hong Fu, Bo Zhang, Xu Wang, Liang Li, Zhongqiu Liu, Di |
author_sort | Ma, Hong |
collection | PubMed |
description | BACKGROUND: The human hematopoietic stem/progenitor cell 117 (HSPC117) protein is involved in many important biological processes. OBJECTIVE: This study was designed to identify the level of HSPC117 mRNA expression in 10 min pig tissue samples and HSPC117 subcellular localization in the PK15 cell line. METHODS: In this study, 10 tissue samples of min pigs were collected, and EGFP-HSPC117 vectors were constructed to express EGFP-HSPC117 fusion proteins in PK15 cells. RESULTS: HSPC117 mRNA was expressed in all of the tissue samples, although the levels of expression in fat and lung tissues were significantly lower than in other tissues ([Formula: see text] 0.01). After generating and detecting the EGFP-HSPC117 fusion protein, fluorescence was found to be distributed throughout the cytoplasm and nucleus during interphase; however, the fluorescence was concentrated in the nuclear area in mitotic cells. CONCLUSIONS: These results indicate that the HSPC117 gene is expressed in many min pig tissues. The HSPC117 protein was distributed throughout the cells during interphase, but was concentrated in the nuclear area in mitotic cells. |
format | Online Article Text |
id | pubmed-6598026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65980262019-07-01 Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line Ma, Hong Fu, Bo Zhang, Xu Wang, Liang Li, Zhongqiu Liu, Di Technol Health Care Research Article BACKGROUND: The human hematopoietic stem/progenitor cell 117 (HSPC117) protein is involved in many important biological processes. OBJECTIVE: This study was designed to identify the level of HSPC117 mRNA expression in 10 min pig tissue samples and HSPC117 subcellular localization in the PK15 cell line. METHODS: In this study, 10 tissue samples of min pigs were collected, and EGFP-HSPC117 vectors were constructed to express EGFP-HSPC117 fusion proteins in PK15 cells. RESULTS: HSPC117 mRNA was expressed in all of the tissue samples, although the levels of expression in fat and lung tissues were significantly lower than in other tissues ([Formula: see text] 0.01). After generating and detecting the EGFP-HSPC117 fusion protein, fluorescence was found to be distributed throughout the cytoplasm and nucleus during interphase; however, the fluorescence was concentrated in the nuclear area in mitotic cells. CONCLUSIONS: These results indicate that the HSPC117 gene is expressed in many min pig tissues. The HSPC117 protein was distributed throughout the cells during interphase, but was concentrated in the nuclear area in mitotic cells. IOS Press 2019-06-18 /pmc/articles/PMC6598026/ /pubmed/31045548 http://dx.doi.org/10.3233/THC-199028 Text en © 2019 – IOS Press and the authors. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/ This article is published online with Open Access and distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC 4.0). |
spellingShingle | Research Article Ma, Hong Fu, Bo Zhang, Xu Wang, Liang Li, Zhongqiu Liu, Di Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title | Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title_full | Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title_fullStr | Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title_full_unstemmed | Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title_short | Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line |
title_sort | expression and subcellular localization of hspc117 in min pig tissues and the pk15 cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598026/ https://www.ncbi.nlm.nih.gov/pubmed/31045548 http://dx.doi.org/10.3233/THC-199028 |
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