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Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis

Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokin...

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Autores principales: Zeng, Jianyan, Zhang, Mi, Hou, Lei, Bai, Wenqin, Yan, Xingying, Hou, Nan, Wang, Hongxing, Huang, Juan, Zhao, Juan, Pei, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598071/
https://www.ncbi.nlm.nih.gov/pubmed/30970146
http://dx.doi.org/10.1093/jxb/erz162
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author Zeng, Jianyan
Zhang, Mi
Hou, Lei
Bai, Wenqin
Yan, Xingying
Hou, Nan
Wang, Hongxing
Huang, Juan
Zhao, Juan
Pei, Yan
author_facet Zeng, Jianyan
Zhang, Mi
Hou, Lei
Bai, Wenqin
Yan, Xingying
Hou, Nan
Wang, Hongxing
Huang, Juan
Zhao, Juan
Pei, Yan
author_sort Zeng, Jianyan
collection PubMed
description Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. In turn, supplementation with auxin can recover fiber growth of CK-treated ovules and mutant ovules. We further found that GhPIN3a is a key auxin transporter for fiber-cell initiation and is polarly localized to the plasma membranes of non-fiber cells, but not to those of fiber cells. This polar localization allows auxin to be transported within the ovule integument while specifically accumulating in fiber cells. We show that CKs antagonize the promotive effect of auxin on fiber cell initiation by undermining asymmetric accumulation of auxin in the ovule epidermis through down-regulation of GhPIN3a and disturbance of the polar localization of the protein.
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spelling pubmed-65980712019-07-03 Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis Zeng, Jianyan Zhang, Mi Hou, Lei Bai, Wenqin Yan, Xingying Hou, Nan Wang, Hongxing Huang, Juan Zhao, Juan Pei, Yan J Exp Bot Research Papers Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. In turn, supplementation with auxin can recover fiber growth of CK-treated ovules and mutant ovules. We further found that GhPIN3a is a key auxin transporter for fiber-cell initiation and is polarly localized to the plasma membranes of non-fiber cells, but not to those of fiber cells. This polar localization allows auxin to be transported within the ovule integument while specifically accumulating in fiber cells. We show that CKs antagonize the promotive effect of auxin on fiber cell initiation by undermining asymmetric accumulation of auxin in the ovule epidermis through down-regulation of GhPIN3a and disturbance of the polar localization of the protein. Oxford University Press 2019-06-01 2019-04-10 /pmc/articles/PMC6598071/ /pubmed/30970146 http://dx.doi.org/10.1093/jxb/erz162 Text en © Society for Experimental Biology 2019. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Papers
Zeng, Jianyan
Zhang, Mi
Hou, Lei
Bai, Wenqin
Yan, Xingying
Hou, Nan
Wang, Hongxing
Huang, Juan
Zhao, Juan
Pei, Yan
Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title_full Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title_fullStr Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title_full_unstemmed Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title_short Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis
title_sort cytokinin inhibits cotton fiber initiation by disrupting pin3a-mediated asymmetric accumulation of auxin in the ovule epidermis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598071/
https://www.ncbi.nlm.nih.gov/pubmed/30970146
http://dx.doi.org/10.1093/jxb/erz162
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