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A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation
The whitefly Bemisia tabaci is an important pest of worldwide agriculture. Previous work has shown that B. tabaci actively suppresses host plant defenses, but our knowledge of the specific mechanisms involved remains limited. Here we describe a B. tabaci salivary protein, the ferritin BtFer1, and it...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598096/ https://www.ncbi.nlm.nih.gov/pubmed/30949671 http://dx.doi.org/10.1093/jxb/erz152 |
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author | Su, Qi Peng, Zhengke Tong, Hong Xie, Wen Wang, Shaoli Wu, Qingjun Zhang, Jianmin Li, Chuanren Zhang, Youjun |
author_facet | Su, Qi Peng, Zhengke Tong, Hong Xie, Wen Wang, Shaoli Wu, Qingjun Zhang, Jianmin Li, Chuanren Zhang, Youjun |
author_sort | Su, Qi |
collection | PubMed |
description | The whitefly Bemisia tabaci is an important pest of worldwide agriculture. Previous work has shown that B. tabaci actively suppresses host plant defenses, but our knowledge of the specific mechanisms involved remains limited. Here we describe a B. tabaci salivary protein, the ferritin BtFer1, and its role in facilitating exploitation of host plants. We show that BtFer1 exhibits Fe(2+) binding ability and ferroxidase activity, and that secretion of BtFer1 during B. tabaci feeding suppresses H(2)O(2)-generated oxidative signals in tomato (Solanum lycopersicum). Silencing BtFer1 enhanced the induction of the jasmonic acid (JA)-mediated defense signaling pathway in response to whitefly feeding, and led to increased callose deposition and the production of proteinase inhibitors that prevent whiteflies from continuously ingesting and digesting phloem sap. Consistent with these effects, silencing BtFer1 reduced whitefly survival on tomato but not on artificial diet. Using a JA-deficient spr2 mutant plant further showed that suppression of JA defenses by BtFer1 is sufficient to increase B. tabaci survival. Taken together, these results demonstrate that BtFer1 acts as an effector protein that mediates whitefly–tomato interactions. These findings represent an important step forward in understanding the molecular mechanisms by which whiteflies and other insect herbivores suppress host plant defenses. |
format | Online Article Text |
id | pubmed-6598096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65980962019-07-03 A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation Su, Qi Peng, Zhengke Tong, Hong Xie, Wen Wang, Shaoli Wu, Qingjun Zhang, Jianmin Li, Chuanren Zhang, Youjun J Exp Bot Research Papers The whitefly Bemisia tabaci is an important pest of worldwide agriculture. Previous work has shown that B. tabaci actively suppresses host plant defenses, but our knowledge of the specific mechanisms involved remains limited. Here we describe a B. tabaci salivary protein, the ferritin BtFer1, and its role in facilitating exploitation of host plants. We show that BtFer1 exhibits Fe(2+) binding ability and ferroxidase activity, and that secretion of BtFer1 during B. tabaci feeding suppresses H(2)O(2)-generated oxidative signals in tomato (Solanum lycopersicum). Silencing BtFer1 enhanced the induction of the jasmonic acid (JA)-mediated defense signaling pathway in response to whitefly feeding, and led to increased callose deposition and the production of proteinase inhibitors that prevent whiteflies from continuously ingesting and digesting phloem sap. Consistent with these effects, silencing BtFer1 reduced whitefly survival on tomato but not on artificial diet. Using a JA-deficient spr2 mutant plant further showed that suppression of JA defenses by BtFer1 is sufficient to increase B. tabaci survival. Taken together, these results demonstrate that BtFer1 acts as an effector protein that mediates whitefly–tomato interactions. These findings represent an important step forward in understanding the molecular mechanisms by which whiteflies and other insect herbivores suppress host plant defenses. Oxford University Press 2019-06-01 2019-04-05 /pmc/articles/PMC6598096/ /pubmed/30949671 http://dx.doi.org/10.1093/jxb/erz152 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Papers Su, Qi Peng, Zhengke Tong, Hong Xie, Wen Wang, Shaoli Wu, Qingjun Zhang, Jianmin Li, Chuanren Zhang, Youjun A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title | A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title_full | A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title_fullStr | A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title_full_unstemmed | A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title_short | A salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
title_sort | salivary ferritin in the whitefly suppresses plant defenses and facilitates host exploitation |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598096/ https://www.ncbi.nlm.nih.gov/pubmed/30949671 http://dx.doi.org/10.1093/jxb/erz152 |
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