Cargando…
Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil
In early 2017, an outbreak caused by an unknown and supposedly viral agent in the Marilena region of southern Brazil was investigated. Since the etiological agent causing the outbreak was not identified from human samples, mosquitoes from this region were collected. Three out of 121 mosquito pools c...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598490/ https://www.ncbi.nlm.nih.gov/pubmed/31237479 http://dx.doi.org/10.1080/22221751.2019.1632152 |
_version_ | 1783430783420071936 |
---|---|
author | Tschá, Marcel Kruchelski Suzukawa, Andreia Akemi Gräf, Tiago Piancini, Laercio Dante Stein da Silva, Allan Martins Faoro, Helisson Riediger, Irina Nastassja Medeiros, Lia Carolina Wowk, Pryscilla Fanini Zanluca, Camila Duarte dos Santos, Claudia Nunes |
author_facet | Tschá, Marcel Kruchelski Suzukawa, Andreia Akemi Gräf, Tiago Piancini, Laercio Dante Stein da Silva, Allan Martins Faoro, Helisson Riediger, Irina Nastassja Medeiros, Lia Carolina Wowk, Pryscilla Fanini Zanluca, Camila Duarte dos Santos, Claudia Nunes |
author_sort | Tschá, Marcel Kruchelski |
collection | PubMed |
description | In early 2017, an outbreak caused by an unknown and supposedly viral agent in the Marilena region of southern Brazil was investigated. Since the etiological agent causing the outbreak was not identified from human samples, mosquitoes from this region were collected. Three out of 121 mosquito pools collected from the region tested positive for alphavirus in molecular tests. Next generation sequencing results revealed the presence of a novel alphavirus, tentatively named here as Caainguá virus (CAAV). DNA barcoding analyses indicated that different species of Culex are hosts for CAAV. This new virus was basal to the New World encephalitic alphaviruses in a comprehensive and robust phylogenetic approach using complete genomes. Viral particles were observed in the cytosol and inside of intracellular compartments of cells in mosquito-derived cell cultures. Despite being noninfectious in vertebrate derived cell cultures, primary culturing of CAAV in human mononuclear cells suggests monocytes and lymphocytes as CAAV targets. However, the epidemiological link of CAAV on the human outbreak should be further explored. |
format | Online Article Text |
id | pubmed-6598490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-65984902019-07-03 Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil Tschá, Marcel Kruchelski Suzukawa, Andreia Akemi Gräf, Tiago Piancini, Laercio Dante Stein da Silva, Allan Martins Faoro, Helisson Riediger, Irina Nastassja Medeiros, Lia Carolina Wowk, Pryscilla Fanini Zanluca, Camila Duarte dos Santos, Claudia Nunes Emerg Microbes Infect Original Articles In early 2017, an outbreak caused by an unknown and supposedly viral agent in the Marilena region of southern Brazil was investigated. Since the etiological agent causing the outbreak was not identified from human samples, mosquitoes from this region were collected. Three out of 121 mosquito pools collected from the region tested positive for alphavirus in molecular tests. Next generation sequencing results revealed the presence of a novel alphavirus, tentatively named here as Caainguá virus (CAAV). DNA barcoding analyses indicated that different species of Culex are hosts for CAAV. This new virus was basal to the New World encephalitic alphaviruses in a comprehensive and robust phylogenetic approach using complete genomes. Viral particles were observed in the cytosol and inside of intracellular compartments of cells in mosquito-derived cell cultures. Despite being noninfectious in vertebrate derived cell cultures, primary culturing of CAAV in human mononuclear cells suggests monocytes and lymphocytes as CAAV targets. However, the epidemiological link of CAAV on the human outbreak should be further explored. Taylor & Francis 2019-06-25 /pmc/articles/PMC6598490/ /pubmed/31237479 http://dx.doi.org/10.1080/22221751.2019.1632152 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Tschá, Marcel Kruchelski Suzukawa, Andreia Akemi Gräf, Tiago Piancini, Laercio Dante Stein da Silva, Allan Martins Faoro, Helisson Riediger, Irina Nastassja Medeiros, Lia Carolina Wowk, Pryscilla Fanini Zanluca, Camila Duarte dos Santos, Claudia Nunes Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title | Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title_full | Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title_fullStr | Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title_full_unstemmed | Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title_short | Identification of a novel alphavirus related to the encephalitis complexes circulating in southern Brazil |
title_sort | identification of a novel alphavirus related to the encephalitis complexes circulating in southern brazil |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598490/ https://www.ncbi.nlm.nih.gov/pubmed/31237479 http://dx.doi.org/10.1080/22221751.2019.1632152 |
work_keys_str_mv | AT tschamarcelkruchelski identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT suzukawaandreiaakemi identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT graftiago identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT piancinilaerciodantestein identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT dasilvaallanmartins identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT faorohelisson identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT riedigeririnanastassja identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT medeirosliacarolina identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT wowkpryscillafanini identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT zanlucacamila identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil AT duartedossantosclaudianunes identificationofanovelalphavirusrelatedtotheencephalitiscomplexescirculatinginsouthernbrazil |