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Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a
BACKGROUND: Ginsenoside Rg3 has been reported to exert protection function on germ cells. However, the mechanisms by which Rg3 regulates apoptosis in mouse Leydig cells remain unclear. In addition, triptolide (TP) has been reported to induce infertility in male rats. Thus, this study aimed to invest...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598939/ https://www.ncbi.nlm.nih.gov/pubmed/31296984 http://dx.doi.org/10.2147/DDDT.S208328 |
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author | Liang, Haiyan Zhang, Suwei Li, Zhiling |
author_facet | Liang, Haiyan Zhang, Suwei Li, Zhiling |
author_sort | Liang, Haiyan |
collection | PubMed |
description | BACKGROUND: Ginsenoside Rg3 has been reported to exert protection function on germ cells. However, the mechanisms by which Rg3 regulates apoptosis in mouse Leydig cells remain unclear. In addition, triptolide (TP) has been reported to induce infertility in male rats. Thus, this study aimed to investigate the protective effect of Rg3 against TP-induced toxicity in MLTC-1 cells. METHODS: CCK-8, immunofluorescence assay, Western blotting and flow cytometry were used to detect cell proliferation and cell apoptosis, respectively. In addition, the dual luciferase reporter system assay was used to detect the interaction between miR-26a and GSK3β in MLTC-1 cells. RESULTS: TP significantly inhibited the proliferation of MLTC-1 cells, while the inhibitory effect of TP was reversed by Rg3. In addition, TP markedly induced apoptosis in MLTC-1 cells via increasing the expressions of Bax, active caspase 3, Cyto c and active caspase 9, and decreasing the level of Bcl-2. However, Rg3 alleviated TP-induced apoptosis of MLTC-1 cells. Moreover, the level of miR-26a was obviously downregulated by Rg3 treatment. The protective effect of Rg3 against TP-induced toxicity in MLTC-1 cells was abolished by miR-26a upregulation. Meanwhile, dual-luciferase assay showed GSK3β was the direct target of miR-26a in MLTC-1 cells. Overexpression of miR-26a markedly decreased the level of GSK3β. As expected, upregulation of miR-26a could abrogate the protective effects of Rg3 against TP-induced cytotoxicity via inhibiting the expression of GSK3β. CONCLUSION: These results indicated that Rg3 could protect MLTC-1 against TP by downregulation of miR-26a. Therefore, Rg3 might serve as a potential agent for the treatment of male hypogonadism. |
format | Online Article Text |
id | pubmed-6598939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-65989392019-07-11 Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a Liang, Haiyan Zhang, Suwei Li, Zhiling Drug Des Devel Ther Original Research BACKGROUND: Ginsenoside Rg3 has been reported to exert protection function on germ cells. However, the mechanisms by which Rg3 regulates apoptosis in mouse Leydig cells remain unclear. In addition, triptolide (TP) has been reported to induce infertility in male rats. Thus, this study aimed to investigate the protective effect of Rg3 against TP-induced toxicity in MLTC-1 cells. METHODS: CCK-8, immunofluorescence assay, Western blotting and flow cytometry were used to detect cell proliferation and cell apoptosis, respectively. In addition, the dual luciferase reporter system assay was used to detect the interaction between miR-26a and GSK3β in MLTC-1 cells. RESULTS: TP significantly inhibited the proliferation of MLTC-1 cells, while the inhibitory effect of TP was reversed by Rg3. In addition, TP markedly induced apoptosis in MLTC-1 cells via increasing the expressions of Bax, active caspase 3, Cyto c and active caspase 9, and decreasing the level of Bcl-2. However, Rg3 alleviated TP-induced apoptosis of MLTC-1 cells. Moreover, the level of miR-26a was obviously downregulated by Rg3 treatment. The protective effect of Rg3 against TP-induced toxicity in MLTC-1 cells was abolished by miR-26a upregulation. Meanwhile, dual-luciferase assay showed GSK3β was the direct target of miR-26a in MLTC-1 cells. Overexpression of miR-26a markedly decreased the level of GSK3β. As expected, upregulation of miR-26a could abrogate the protective effects of Rg3 against TP-induced cytotoxicity via inhibiting the expression of GSK3β. CONCLUSION: These results indicated that Rg3 could protect MLTC-1 against TP by downregulation of miR-26a. Therefore, Rg3 might serve as a potential agent for the treatment of male hypogonadism. Dove 2019-06-24 /pmc/articles/PMC6598939/ /pubmed/31296984 http://dx.doi.org/10.2147/DDDT.S208328 Text en © 2019 Liang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Liang, Haiyan Zhang, Suwei Li, Zhiling Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title | Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title_full | Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title_fullStr | Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title_full_unstemmed | Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title_short | Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a |
title_sort | ginsenoside rg3 protects mouse leydig cells against triptolide by downregulation of mir-26a |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598939/ https://www.ncbi.nlm.nih.gov/pubmed/31296984 http://dx.doi.org/10.2147/DDDT.S208328 |
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