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Sesame oleosins are minor allergens
BACKGROUND: In daily practice, one-third of sesame allergic patients, confirmed by clinical history or food challenge, do not show any detectable specific IgE using current diagnostics. Currently used sesame extracts are water-based and therefore lacking hydrophobic proteins like oleosins. Oleosins,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599271/ https://www.ncbi.nlm.nih.gov/pubmed/31297180 http://dx.doi.org/10.1186/s13601-019-0271-x |
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author | Ehlers, Anna M. Rossnagel, Madlen Brix, Bettina Blankestijn, Mark A. Le, Thuy-My Suer, Waltraud Otten, Henny G. Knulst, André C. |
author_facet | Ehlers, Anna M. Rossnagel, Madlen Brix, Bettina Blankestijn, Mark A. Le, Thuy-My Suer, Waltraud Otten, Henny G. Knulst, André C. |
author_sort | Ehlers, Anna M. |
collection | PubMed |
description | BACKGROUND: In daily practice, one-third of sesame allergic patients, confirmed by clinical history or food challenge, do not show any detectable specific IgE using current diagnostics. Currently used sesame extracts are water-based and therefore lacking hydrophobic proteins like oleosins. Oleosins, the stabilizer of lipid droplets in plants, are described as allergens in sesame, peanut and hazelnut. In this study, we examine the role of oleosins in sesame allergy and their potential cross-reactivity between sesame and (pea)nuts. METHODS: Specific IgE and IgG sensitisation to native and heterologously expressed sesame components and oleosins from other nuts, free of seed storage proteins, was assessed by line blot and sera from 17 sesame allergic patients without detectable specific IgE sensitisation to sesame, and compared to 18 sesame allergic and 13 tolerant patients with specific IgE sensitisation to sesame. RESULTS: Sesame allergic patients without sensitisation showed no specific IgE to the tested sesame oleosins or components. Low levels of specific IgE to sesame oleosins were detected in 17% of sesame allergic and 15% of tolerant patients with sIgE sensitisation. Oleosins were recognised by serum IgG from multiple patients confirming immune reactivity and excluding technical issues leading to lack of specific IgE-binding to oleosins. CONCLUSION: Sesame oleosins are minor allergens and appear to have no additonal value in diagnosing sesame allergy in adults based on sIgE and sIgG detection. There is a high need for additional diagnostic tools in those patients to minimize the number of required food challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13601-019-0271-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6599271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65992712019-07-11 Sesame oleosins are minor allergens Ehlers, Anna M. Rossnagel, Madlen Brix, Bettina Blankestijn, Mark A. Le, Thuy-My Suer, Waltraud Otten, Henny G. Knulst, André C. Clin Transl Allergy Letter to the Editor BACKGROUND: In daily practice, one-third of sesame allergic patients, confirmed by clinical history or food challenge, do not show any detectable specific IgE using current diagnostics. Currently used sesame extracts are water-based and therefore lacking hydrophobic proteins like oleosins. Oleosins, the stabilizer of lipid droplets in plants, are described as allergens in sesame, peanut and hazelnut. In this study, we examine the role of oleosins in sesame allergy and their potential cross-reactivity between sesame and (pea)nuts. METHODS: Specific IgE and IgG sensitisation to native and heterologously expressed sesame components and oleosins from other nuts, free of seed storage proteins, was assessed by line blot and sera from 17 sesame allergic patients without detectable specific IgE sensitisation to sesame, and compared to 18 sesame allergic and 13 tolerant patients with specific IgE sensitisation to sesame. RESULTS: Sesame allergic patients without sensitisation showed no specific IgE to the tested sesame oleosins or components. Low levels of specific IgE to sesame oleosins were detected in 17% of sesame allergic and 15% of tolerant patients with sIgE sensitisation. Oleosins were recognised by serum IgG from multiple patients confirming immune reactivity and excluding technical issues leading to lack of specific IgE-binding to oleosins. CONCLUSION: Sesame oleosins are minor allergens and appear to have no additonal value in diagnosing sesame allergy in adults based on sIgE and sIgG detection. There is a high need for additional diagnostic tools in those patients to minimize the number of required food challenges. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13601-019-0271-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-28 /pmc/articles/PMC6599271/ /pubmed/31297180 http://dx.doi.org/10.1186/s13601-019-0271-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Letter to the Editor Ehlers, Anna M. Rossnagel, Madlen Brix, Bettina Blankestijn, Mark A. Le, Thuy-My Suer, Waltraud Otten, Henny G. Knulst, André C. Sesame oleosins are minor allergens |
title | Sesame oleosins are minor allergens |
title_full | Sesame oleosins are minor allergens |
title_fullStr | Sesame oleosins are minor allergens |
title_full_unstemmed | Sesame oleosins are minor allergens |
title_short | Sesame oleosins are minor allergens |
title_sort | sesame oleosins are minor allergens |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599271/ https://www.ncbi.nlm.nih.gov/pubmed/31297180 http://dx.doi.org/10.1186/s13601-019-0271-x |
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