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Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study

Background As chronic kidney disease (CKD) is a silent killer, it is now a global concern to find out the possible causes by genetic and biological markers. In the earlier stages of CKD, serum calcium and phosphorus levels fall down later on turned into hypercalcemia and hyperphosphatemia contributi...

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Autores principales: Jahan, Ishrat, Ahmed, Salma, Islam, Md Rabiul, Hai, Abu Noim Md Abdul, Islam, Mohammad Faijul, Bhuiyan, Mohiuddin Ahmed, Nahar, Zabun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599462/
https://www.ncbi.nlm.nih.gov/pubmed/31281748
http://dx.doi.org/10.7759/cureus.4564
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author Jahan, Ishrat
Ahmed, Salma
Islam, Md Rabiul
Hai, Abu Noim Md Abdul
Islam, Mohammad Faijul
Bhuiyan, Mohiuddin Ahmed
Nahar, Zabun
author_facet Jahan, Ishrat
Ahmed, Salma
Islam, Md Rabiul
Hai, Abu Noim Md Abdul
Islam, Mohammad Faijul
Bhuiyan, Mohiuddin Ahmed
Nahar, Zabun
author_sort Jahan, Ishrat
collection PubMed
description Background As chronic kidney disease (CKD) is a silent killer, it is now a global concern to find out the possible causes by genetic and biological markers. In the earlier stages of CKD, serum calcium and phosphorus levels fall down later on turned into hypercalcemia and hyperphosphatemia contributing high mortality in CKD. This study aimed to examine the serum calcium and phosphorus levels in non-dialysis CKD patients and healthy controls to find out their association with ORAI1 genetic polymorphism. Methods The present study recruited 96 non-dialysis CKD patients and 100 control subjects matched by age, gender, and body mass index (BMI). Measurement of serum calcium levels was performed with atomic absorption spectrophotometer (HITACHI, 170-30) and phosphorus levels were determined by UV VIS spectrophotometer (Analytik Jena SPEKOL 2000). PCR-RFLP technique was applied to determine the genetic polymorphism of ORAI1 (rs12313273 and rs6486795) gene. Results The mean values of serum calcium and phosphorus levels were 2.53 ± 0.50 mg/dL and 3.77 ± 0.42 mg/dL for the patient group and 3.67 ± 2.37 mg/dL and 13.66 ± 6.34 mg/dL for the control group, respectively. We observed significantly reduced serum calcium and phosphorus levels in non-dialysis CKD patients compared with control subjects (p < 0.001). No significant polymorphism of ORAI1 (rs12313273 and rs6486795) was found with declined serum calcium and phosphorus levels. Conclusions The present study suggested that there is no linear correlation between ORAI1 genetic polymorphism with serum calcium and phosphorus levels in non-dialysis CKD patients.
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spelling pubmed-65994622019-07-05 Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study Jahan, Ishrat Ahmed, Salma Islam, Md Rabiul Hai, Abu Noim Md Abdul Islam, Mohammad Faijul Bhuiyan, Mohiuddin Ahmed Nahar, Zabun Cureus Genetics Background As chronic kidney disease (CKD) is a silent killer, it is now a global concern to find out the possible causes by genetic and biological markers. In the earlier stages of CKD, serum calcium and phosphorus levels fall down later on turned into hypercalcemia and hyperphosphatemia contributing high mortality in CKD. This study aimed to examine the serum calcium and phosphorus levels in non-dialysis CKD patients and healthy controls to find out their association with ORAI1 genetic polymorphism. Methods The present study recruited 96 non-dialysis CKD patients and 100 control subjects matched by age, gender, and body mass index (BMI). Measurement of serum calcium levels was performed with atomic absorption spectrophotometer (HITACHI, 170-30) and phosphorus levels were determined by UV VIS spectrophotometer (Analytik Jena SPEKOL 2000). PCR-RFLP technique was applied to determine the genetic polymorphism of ORAI1 (rs12313273 and rs6486795) gene. Results The mean values of serum calcium and phosphorus levels were 2.53 ± 0.50 mg/dL and 3.77 ± 0.42 mg/dL for the patient group and 3.67 ± 2.37 mg/dL and 13.66 ± 6.34 mg/dL for the control group, respectively. We observed significantly reduced serum calcium and phosphorus levels in non-dialysis CKD patients compared with control subjects (p < 0.001). No significant polymorphism of ORAI1 (rs12313273 and rs6486795) was found with declined serum calcium and phosphorus levels. Conclusions The present study suggested that there is no linear correlation between ORAI1 genetic polymorphism with serum calcium and phosphorus levels in non-dialysis CKD patients. Cureus 2019-04-29 /pmc/articles/PMC6599462/ /pubmed/31281748 http://dx.doi.org/10.7759/cureus.4564 Text en Copyright © 2019, Jahan et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Genetics
Jahan, Ishrat
Ahmed, Salma
Islam, Md Rabiul
Hai, Abu Noim Md Abdul
Islam, Mohammad Faijul
Bhuiyan, Mohiuddin Ahmed
Nahar, Zabun
Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title_full Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title_fullStr Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title_full_unstemmed Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title_short Association of ORAI1 Genetic Polymorphism with Serum Calcium and Phosphorus Levels in Non-dialysis Chronic Kidney Disease Patients: A Case-control Study
title_sort association of orai1 genetic polymorphism with serum calcium and phosphorus levels in non-dialysis chronic kidney disease patients: a case-control study
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599462/
https://www.ncbi.nlm.nih.gov/pubmed/31281748
http://dx.doi.org/10.7759/cureus.4564
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